Structural and spectroscopic studies on the formation of lipoplexes between DNA and cationic gemini surfactants

被引：8
作者：
Andrzejewska, Weronika [1 ]
Pietralik, Zuzanna [1 ]
Taube, Michal [1 ]
Skrzypczak, Andrzej [2 ]
Kozak, Maciej [1 ]
机构：
[1] Adam Mickiewicz Univ, Dept Macromol Phys, Poznan, Poland
[2] Poznan Univ Tech, Fac Chem Technol, Poznan, Poland
来源：
POLIMERY | 2014年 / 59卷 / 7-8期
关键词：
gene therapy; gemini surfactants; DNA; circular dichroism; gel electrophoresis; COMPLEXES; SYSTEMS; TRANSFECTION; CARRIERS; CHLORIDE;
D O I：
10.14314/polimery.2014.569
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
The process of complex formation between cationic gemini surfactants, 3,3'-[alpha,omega-(dioxaalkane)]bis(1-dodecylimidazolium) dichloride, with deoxyribonucleic acid (DNA) was studied. The study was performed for ten surfactants having spacer groups of different lengths used in 6 concentrations (5 mM, 2 mM, 1 mM, 0.5 mM, 0.2 mM, 0.1 mM) and a 6.5 mu M DNA solution. The complex formation was verified by circular dichroism spectroscopy and gel electrophoresis. The complexes were found to be stable and the process of complex formation was reproducible, efficient and immediate.
引用
收藏
页码：569 / 574
页数：6
相关论文
共 22 条
  • [1] Conformational Changes in DNA upon Ligand Binding Monitored by Circular Dichroism
    Chang, Yu-Ming
    Chen, Cammy K. -M.
    Hou, Ming-Hon
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2012, 13 (03): : 3394 - 3413
  • [2] Cationic lipid nanosystems as carriers for nucleic acids
    Cortesi, Rita
    Campioni, Matteo
    Ravani, Laura
    Drechsler, Markus
    Pinotti, Mirko
    Esposito, Elisabetta
    [J]. NEW BIOTECHNOLOGY, 2014, 31 (01) : 44 - 54
  • [3] Building blocks for ionic liquids: Vapor pressures and vaporization enthalpies of 1-(n-alkyl)-imidazoles
    Emel'yanenko, Vladimir N.
    Portnova, Svetlana V.
    Verevkin, Sergey P.
    Skrzypczak, Andrzej
    Schubert, Thomas
    [J]. JOURNAL OF CHEMICAL THERMODYNAMICS, 2011, 43 (10) : 1500 - 1505
  • [4] Cationic lipid-DNA complexes for gene therapy: Understanding the relationship between complex structure and gene delivery pathways at the molecular level
    Ewert, K
    Slack, NL
    Ahmad, A
    Evans, HM
    Lin, AJ
    Samuel, CE
    Safinya, CR
    [J]. CURRENT MEDICINAL CHEMISTRY, 2004, 11 (02) : 133 - 149
  • [5] Hait SK, 2002, CURR SCI INDIA, V82, P1101
  • [6] Compaction of DNA by gemini surfactants:: Effects of surfactant architecture
    Karlsson, L
    van Eijk, MCP
    Söderman, O
    [J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2002, 252 (02) : 290 - 296
  • [7] Gemini surfactants:: New synthetic vectors for gene transfection
    Kirby, AJ
    Camilleri, P
    Engberts, JBFN
    Feiters, MC
    Nolte, RJM
    Söderman, O
    Bergsma, M
    Bell, PC
    Fielden, ML
    Rodríguez, CLG
    Guédat, P
    Kremer, A
    McGregor, C
    Perrin, C
    Ronsin, G
    van Eijk, MCP
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (13) : 1448 - 1457
  • [8] Bio-nano complexes: DNA/surfactant/single-walled carbon nanotube interactions in electric field
    Lee, HyungKi
    Mijovic, Jovan
    [J]. POLYMER, 2009, 50 (03) : 881 - 890
  • [9] Influence of the spacer of cationic gemini amphiphiles on the hydration of lipoplexes
    Luciani, Paola
    Bombelli, Cecilia
    Colone, Marisa
    Giansanti, Luisa
    Ryhanen, Samppa J.
    Saily, V. Matti J.
    Mancini, Giovanna
    Kinnunen, Paavo K. J.
    [J]. BIOMACROMOLECULES, 2007, 8 (06) : 1999 - 2003
  • [10] Long-circulating DNA lipid nanocapsules as new vector for passive tumor targeting
    Morille, Marie
    Montier, Tristan
    Legras, Pierre
    Carmoy, Nathalie
    Brodin, Priscille
    Pitard, Bruno
    Benoit, Jean-Pierre
    Passirani, Catherine
    [J]. BIOMATERIALS, 2010, 31 (02) : 321 - 329
123