Genetic diversity of bovine ulcerative mammary dermatitis-associated Treponema

被引：31
作者：
Stamm, L. V. [1 ]
Walker, R. L. [2 ]
Read, D. H. [3 ]
机构：
[1] Univ N Carolina, Dept Epidemiol, Program Infect Dis, Gillings Sch Global Publ Hlth, Chapel Hill, NC 27599 USA
[2] Univ Calif Davis, Calif Anim Hlth & Food Safety Lab, Davis Branch, Sch Vet Med, Davis, CA 95616 USA
[3] Univ Calif Davis, Calif Anim Hlth & Food Safety Lab, San Bernardino Branch, Sch Vet Med, San Bernardino, CA 92408 USA
来源：
VETERINARY MICROBIOLOGY | 2009年 / 136卷 / 1-2期
关键词：
Bovine udder lesions; Ulcerative mammary dermatitis; Papillomatous digital dermatitis; Treponema; 16S-23S rDNA intergenic spacer region; PAPILLOMATOUS DIGITAL DERMATITIS; DAIRY-CATTLE; LESIONS; PERIODONTITIS;
D O I：
10.1016/j.vetmic.2008.10.022
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The etiology of bovine ulcerative mammary dermatitis (UMD) is poorly characterized. The goal of this study was to genetically analyze spirochetes present in UMD lesions. DNA prepared from UMD lesion biopsies and from spirochetes cultured from the corresponding lesion biopsies was PCR amplified using primers for the 16S rDNA-tRNA(ile) intergenic spacer region (ISR) of Treponema 16S-23S rDNA. Analysis of cloned ISR amplicons from three cultivable UMD-associated spirochetes indicated that two isolates cluster closely with cultivable papillomatous digital dermatitis (PDD)-associated and human-associated Treponema phylotypes, while the remaining isolate is unique. Analysis of ISR amplicons from UMD lesion biopsies identified additional not-yet-cultivable Treponema phylotypes. Our results revealed the presence of a genetically diverse Treponema population in an UMD lesion. (C) 2008 Elsevier B.V. All rights reserved.
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收藏
页码：192 / 196
页数：5
相关论文
共 19 条
  • [1] BEATTIE KG, 2000, J BRIT CATTLE VET AS, V8, P377
  • [2] BLOWEY R, 1995, MASTITIS CONTROL DAI, P169
  • [3] Boyer P, 1998, VET REC, V142, P147
  • [4] DIVERSITY OF CULTIVABLE AND UNCULTIVABLE ORAL SPIROCHETES FROM A PATIENT WITH SEVERE DESTRUCTIVE PERIODONTITIS
    CHOI, BK
    PASTER, BJ
    DEWHIRST, FE
    GOBEL, UB
    [J]. INFECTION AND IMMUNITY, 1994, 62 (05) : 1889 - 1895
  • [5] Spirochetes from digital dermatitis lesions in cattle are closely related to treponemes associated with human periodontitis
    Choi, BK
    Nattermann, H
    Grund, S
    Haider, W
    Gobel, UB
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1997, 47 (01): : 175 - 181
  • [6] From tooth to hoof: treponemes in tissue-destructive diseases
    Edwards, AM
    Dymock, D
    Jenkinson, HF
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2003, 94 (05) : 767 - 780
  • [7] Lesion formation and antibody response induced by papillomatous digital dermatitis-associated Spirochetes in a murine abscess model
    Elliott, Margaret K.
    Alt, David P.
    Zuerner, Richard L.
    [J]. INFECTION AND IMMUNITY, 2007, 75 (09) : 4400 - 4408
  • [8] Three unique groups of spirochetes isolated from digital dermatitis lesions in UK cattle
    Evans, Nicholas J.
    Brown, Jennifer M.
    Demirkan, Ibrahim
    Murray, Richard D.
    Vink, W. Daan
    Blowey, Roger W.
    Hart, C. Anthony
    Carter, Stuart D.
    [J]. VETERINARY MICROBIOLOGY, 2008, 130 (1-2) : 141 - 150
  • [9] Keil D. J., 2002, P 83 ANN M C RES WOR
  • [10] Evidence of multiple Treponema phylotypes involved in bovine digital dermatitis as shown by 16S rRNA gene analysis and fluorescence in situ hybridization
    Klitgaard, Kirstine
    Boye, Mette
    Capion, Nynne
    Jensen, Tim K.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (09) : 3012 - 3020
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