Water-Soluble Triarylboron Compound for ATP Imaging In Vivo Using Analyte-Induced Finite Aggregation

被引：124

作者：

Li, Xiaoyan ^{[1
]}

Guo, Xudong ^{[1
]}

Cao, Lixia ^{[1
]}

Xun, Zhiqing ^{[2
]}

Wang, Shuangqing ^{[1
]}

Li, Shayu ^{[1
]}

Li, Yi ^{[2
]}

Yang, Guoqiang ^{[1
]}

机构：

[1] Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Photochem, Inst Chem, Beijing 100190, Peoples R China

[2] Chinese Acad Sci, Key Lab Photochem Convers & Optoelect Mat, Tech Inst Phys & Chem, Beijing 100190, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2014年 / 53卷 / 30期

基金：

中国国家自然科学基金;

关键词：

aggregation; cell recognition; fluorescence; imaging agents; molecular recognition; INDUCED EMISSION; RATIONAL DESIGN; SOLID-STATE; FLUORESCENT; NANOPARTICLES; SENSORS; PH;

D O I：

10.1002/anie.201403918

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Adenosine 5'-triphosphate (ATP) is a multifunctional molecule that participates in many important biological processes. Currently, fluorescence indicators for ATP with high performance are in demand. Reported herein is a novel water-soluble triarylboron compound which displays an apparent ATP-dependent fluorescence enhancement when dispersed in water. It can selectively recognize ATP from other bioactive substances in vitro and in vivo. The ATP-induced finite aggregation endows the indicator with appreciable photostability and superior tolerance to environmental electrolytes. This indicator has been successfully applied to the ATP imaging in NIH/3T3 fibroblast cells. The difference in the ATP levels within the membrane and cytosol is clearly visible.

引用

页码：7809 / 7813

页数：5

共 44 条

[11] A Triarylboron-Based Fluorescent Thermometer: Sensitive Over a Wide Temperature Range [J].

Feng, Jiao ;

Tian, Kaijun ;

Hu, Dehui ;

Wang, Shuangqing ;

Li, Shayu ;

Zeng, Yi ;

Li, Yi ;

Yang, Guoqiang .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 50 (35) :8072-8076

[12] Determination of adenosine phosphate by co-electroosmotic capillary electrophoresis with amperometric detection [J].

Fu, CG ;

Song, LN ;

Fang, YZ .

ANALYTICA CHIMICA ACTA, 1999, 399 (03) :259-263

[13] Novel method for measurement of submembrane ATP concentration [J].

Gribble, FM ;

Loussouarn, G ;

Tucker, SJ ;

Zhao, C ;

Nichols, CG ;

Ashcroft, FM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (39) :30046-30049

[14] The machinery of mitochondrial fusion, division, and distribution, and emerging connections to apoptosis [J].

Hales, KG .

MITOCHONDRION, 2004, 4 (04) :285-308

[15] A FAMILY OF RELATED ATP-BINDING SUBUNITS COUPLED TO MANY DISTINCT BIOLOGICAL PROCESSES IN BACTERIA [J].

HIGGINS, CF ;

HILES, ID ;

SALMOND, GPC ;

GILL, DR ;

DOWNIE, JA ;

EVANS, IJ ;

HOLLAND, IB ;

GRAY, L ;

BUCKEL, SD ;

BELL, AW ;

HERMODSON, MA .

NATURE, 1986, 323 (6087) :448-450

[16] Aggregation-induced emission [J].

Hong, Yuning ;

Lam, Jacky W. Y. ;

Tang, Ben Zhong .

CHEMICAL SOCIETY REVIEWS, 2011, 40 (11) :5361-5388

[17] Molecular details of cAMP generation in mammalian cells: A tale of two systems [J].

Kamenetsky, Margarita ;

Middelhaufe, Sabine ;

Bank, Erin M. ;

Levin, Lonny R. ;

Buck, Jochen ;

Steegborn, Clemens .

JOURNAL OF MOLECULAR BIOLOGY, 2006, 362 (04) :623-639

[18]

Khan M.M., 1962, J PHYS CHEM-US, V66, P10

[19]

Kim J. H., 2010, ANGEW CHEM, V122, P1498

[20] A Luciferase/Single-Walled Carbon Nanotube Conjugate for Near-Infrared Fluorescent Detection of Cellular ATP [J].

Kim, Jong-Ho ;

Ahn, Jin-Ho ;

Barone, Paul W. ;

Jin, Hong ;

Zhang, Jingqing ;

Heller, Daniel A. ;

Strano, Michael S. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (08) :1456-1459

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