Thin layer chromatographical detection of tyrosine produced from L-[U-14C]phenylalanine by ruminal microbes

被引：0

作者：

Khan, RI ^{[1
]}

Onodera, R

Amin, MR

机构：

[1] Bangladesh Agr Univ, Fac Anim Husbandry, Dept Anim Sci, Mymensingh 2202, Bangladesh

[2] Miyazaki Univ, Fac Agr, Dept Anim Nutr & Biochem, Miyazaki 88921, Japan

来源：

AMINO ACIDS | 2002年 / 22卷 / 04期

关键词：

amino acids; tyrosine; phenylalanine; rumen bacteria; protozoa;

D O I：

10.1007/s007260200026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thin layer chromatographical detection of tyrosine (Tyr) synthesized from L-[U-C-14]phenylalanine (Phe) (1 mM) by rumen bacteria (B) and protozoa (P) collected from fistulated Japanese Goat was carried out. About 16 and 12% of the added Phe was converted to Tyr by B and P, respectively. Large amount of radioactivity in ether fractions indicated an abundant production of aromatic acids from Phe. Small amount of radioactivity found in CO2 fractions implied an occurrence of considerable decarboxylation reaction(s) by rumen bacteria and protozoa.

引用

页码：427 / 432

页数：6