SYNTHESIS, CHARACTERIZATION, ANTIBACTERIAL AND CYTOTOXIC EFFECTS OF SILVER NANOPARTICLES

To investigate the biomedical potential of silver nanoparticles prepared by reduction of silver ions with ascorbic acid in the presence of the stabilizer Daxad on different pathogenic bacteria and toxicity against human acute promyelocytic leukemia (HL-60) cell line were performed. Characterization using UV-VIS spectrophotometery and X- ray diffraction analysis were used. Antimicrobial activity against five bacterial strains was tested using disc diffusion method and by agar plate and broth medium methods. While, cytotoxicity test using MTT was obtained on the human leukemia cell line (HL-60). UV-Vis spectral analysis showed silver surface plasmon resonance band at 425 nm. X- ray diffraction showed that the particles were crystalline in nature with face centered cubic structure of the bulk silver with broad beaks at 38.56 degrees and 44.81 degrees. In conclusion, the synthesized silver nanoparticles efficiently inhibited the growth of pathogenic bacteria and reduce viability of the HL-60 cells in a dose-dependent manner. Further studies should be given special attention for anticancer potentials while warranting for toxicity against normal cells is needed.