Chryseobacterium formosus sp nov., a bacterium isolated from an ancient tree trunk

A Gram-reaction-negative, non-motile and rod-shaped bacterium, designated as THG-DN3.6(T), was isolated from an ancient tree trunk from Republic of Korea. On the basis of 16S rRNA gene sequence analysis, strain THG-DN3.6(T) was shown to belong to the genus Chryseobacterium and the highest similarity to Chryseobacterium indoltheticum LMG 4025(T) (97.2 %) and the closest phylogenetic relatives were Chryseobacterium scophthalmum (96.8 %), Chryseobacterium piscium (96.7 %) and Chryseobacterium balustinum KCTC 2903(T) (96.3 %). The DNA G + C content of the isolate was 33.2 mol %. The predominant isoprenoid quinone was menaquinone-6. The major fatty acids were iso-C-15:0, summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 7t and/or iso-C-15:0 2-OH), iso-C-17:1 omega 9c and iso-C-17:0 3-OH. The major polar lipids of strain THG-DN3.6(T) were phosphatidylethanolamine. The mean DNA-DNA relatedness of strain THG-DN3.6(T) to C. indoltheticum LMG 4025(T) was 52 +/- A 0.5 %. Based on the results of polyphasic characterization, strain THG-DN3.6(T) represented a novel species within the genus Chryseobacterium, for which the name Chryseobacterium formosus sp. nov. is proposed. The type strain is THG-DN3.6(T) (=KCTC 42606 = CCTCC AB 2015118). The NCBI GenBank accession number for the 16S rRNA gene sequence of strain THG-DN3.6(T) is KM035938.