Galactosylated electrospun membranes for hepatocyte sandwich culture

被引：11
作者：
Chien, Hsiu-Wen [1 ]
Lai, Juin-Yih [2 ,3 ]
Tsai, Wei-Bor [1 ]
机构：
[1] Natl Taiwan Univ, Dept Chem Engn, Taipei 115, Taiwan
[2] Chung Yuan Christian Univ, R&D Ctr Membrane Technol, Taoyuan, Taiwan
[3] Chung Yuan Christian Univ, Dept Chem Engn, Taoyuan, Taiwan
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2014年 / 116卷
关键词：
Electrospun membrane; Polyelectrolyte multilayer deposition; Sandwich culture; Hepatocytes; POLYMER NANOFIBERS; PLATELET-ADHESION; CELL-ADHESION; LIVER; POLYSTYRENE; MODULATION; ADSORPTION; FIBRINOGEN; MORPHOLOGY; SPHEROIDS;
D O I：
10.1016/j.colsurfb.2014.01.040
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
In this work, we developed a galactocylated electrospun polyurethane membrane for sandwich culture of hepatocyte sandwich culture. The electrospun fibrous membranes were bio-functionalized with galactose molecules by a UV-crosslinked layer-by-layer polyelectrolyte multilayer deposition technique. The galactosylated electrospun membranes were employed as a top support membrane for the sandwich culture of HepG2/C3A cells on a collagen substrate. Our results demonstrate that HepG2/C3A cells covered by the galactosylated PU membranes form multi-cellular aggregates and lead to improved albumin secretion ability compared to the control membranes (unmodified PU or poly(ethylene imine)-modified PU). Our study reveals the potential of galactosylated electrospun membranes in the application of liver tissue engineering and the regeneration of liver-tissue substitutes. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：576 / 581
页数：6
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