Involvement of steroids in anti-inflammatory effects of PK11195 in a murine model of pleurisy

被引：12
作者：
da Silva, MBS
Farges, RC
Fröde, TS
机构：
[1] Univ Fed Santa Catarina, Ctr Hlth Sci, Dept Clin Anal, BR-88040970 Florianopolis, SC, Brazil
[2] Univ Fed Santa Catarina, Ctr Biol Sci, Dept Pharmacol, BR-88040970 Florianopolis, SC, Brazil
来源：
MEDIATORS OF INFLAMMATION | 2004年 / 13卷 / 02期
关键词：
PK11195; pleurisy; nitric oxide; steroid synthesis;
D O I：
10.1080/09629350410001688486
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
BACKGROUND: Studies on peripheral benzodiazepine receptor function have yielded a diverse list of activities of which the anti-inflammatory effects need to be further examined. Aims: To evaluate the role of steroids, nitric oxide and adenosine-deaminase in the anti-inflammatory effect of PK11195. Methods: Pleurisy was induced by intrapleural injection of carrageenan in mice pre-treated or not with PK11195. Leukocytes, exudation, adenosine-deaminase (ADA) activity and nitric oxide ( NO) level were measured. Steroid involvement was evaluated by pretreatment with D, L-aminogluthetimide before PK11195. Results: Leukocytes, exudation and NO levels were reduced by PK11195 in the early ( 4 h) phase. In the late ( 48 h) phase, PK11195 decreased leukocytes and ADA activity. D, L-aminogluthetimide reversed the effect of PK11195 on exudate ( 4 h), as well as total and differential leukocytes and NO levels ( 48 h). Conclusions: Steroids, NO and ADA are implicated in the anti-inflammatory action of PK11195.
引用
收藏
页码：93 / 103
页数：11
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