The essential role of clathrin-mediated 'endocytosis in yellow head virus propagation in the black tiger shrimp Penaeus monodon

被引：24
作者：
Jatuyosporn, Thapanan [1 ]
Supungul, Premruethai [1 ,2 ]
Tassanakajon, Anchalee [1 ]
Krusong, Kuakarun [1 ]
机构：
[1] Chulalongkorn Univ, Dept Biochem, Ctr Excellence Mol Biol & Genom Shrimp, Fac Sci, Bangkok 10330, Thailand
[2] NSTDA, Natl Ctr Genet Engn & Biotechnol BIOTEC, Pathum Thani 12120, Thailand
来源：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2014年 / 44卷 / 01期
关键词：
Clathrin coat assembly protein 17; Clathrin-mediated endocytosis; Penaeus monodon; Yellow head virus; YHV; DENGUE-VIRUS; YHV INFECTION; DC-SIGN; ENTRY; IDENTIFICATION; RECEPTOR; GLYCOPROTEINS; PREDICTION; NIDOVIRUS; MEMBRANE;
D O I：
10.1016/j.dci.2013.11.017
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Yellow head virus (YHV) is one of the most widespread viruses seriously affecting black tiger shrimp (Penaeus monodon) cultivation. A previous microarray study demonstrated that clathrin coat assembly protein 17 (API 7) was significantly up-regulated after YHV infection (Pongsomboon et al., 2011). Clathrin coat API 7 is a part of the assembly protein c5.2 (AP-2) complex which is involved in clathrin-mediated endocytosis. Quantitative RT-PCR (qRT-PCR) revealed that the clathrin coat API 7 gene was up-regulated 3-fold at 12 h post YHV infection. In addition, immunofluorescence microscopy showed that clathrin coat API 7 was highly expressed in the cytoplasm of the YHV-infected hemocytes. Knockdown of the clathrin coat API 7 gene dramatically reduced YHV replicativity by 32-fold. Interestingly, shrimp pre-treated with chlorpromazine, a commercial drug that inhibits clathrin-dependent endocytosis, exhibited significantly low levels of YHV infection. Taken together, these results suggest that clathrin-mediated endocytosis is involved in YHV propagation in P. monodon. (C) 2013 Elsevier Ltd. All rights reserved.
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收藏
页码：100 / 110
页数：11
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