The effect of electroporation on iontophoretic transdermal delivery of calcium regulating hormones

被引:65
作者
Chang, SL
Hofmann, GA
Zhang, L
Deftos, LJ
Banga, AK
机构
[1] Mercer Univ, Sch Pharm, Dept Pharmaceut Sci, Atlanta, GA 30341 USA
[2] Auburn Univ, Sch Pharm, Dept Pharmacal Sci, Auburn, AL 36849 USA
[3] Genetron Inc, San Diego, CA 92121 USA
[4] Univ Calif San Diego, La Jolla, CA 92161 USA
[5] San Diego VA Med Ctr, La Jolla, CA 92161 USA
关键词
iontophoresis; electroporation; calcitonin; parathyroid hormone; skin transport;
D O I
10.1016/S0168-3659(99)00262-X
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Electrically-assisted delivery by iontophoresis and/or electroporation was used in vitro to deliver the calcium regulating hormones, salmon calcitonin (sCT) and parathyroid hormone (1-34) (PTH) through human epidermis. Such delivery could be useful for chronic treatment of post-menopausal osteoporosis and other clinical indications as a superior alternative to parenteral delivery, sCT (50 mu g/ml) or PTH (1-34) (100 mu g/ml) formulation was prepared in citrate buffer (pH 4.0 or 5.0, respectively). Epidermis separated from human cadaver skin was used, Iontophoresis was applied using a constant current power source and electroporation with an exponential pulse generator. Silver/silver chloride electrodes were used. A combination of electroporation and iontophoresis resulted in higher transdermal permeation than either one technique alone. Electroporation also shortened the lag time of iontophoretic transdermal delivery of salmon calcitonin. Pulsing at lower voltages followed by iontophoresis did not result in increased transport (over iontophoresis alone), perhaps because the transdermal voltage was very low. The transdermal transport of salmon calcitonin by pulsing with 15 pulses (1 ppm) of 500 V (200 ms) followed by iontophoresis led to a quick input and high flux. The average transdermal voltage was only about 50 V for a 500 V study. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:127 / 133
页数:7
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