FOXC2 is overexpressed and participates in cell proliferation in ovarian cancer

被引:1
|
作者
Quan, Yuan [1 ]
Quan, Zhe [2 ]
Zheng, Chunhua [1 ]
Deng, Weiguo [3 ]
Meng, Fanhui [1 ]
Fu, Yan [1 ]
机构
[1] Jilin Univ, Hosp 1, Dept Gen Gynecol, 71 Xinmin St, Changchun 130021, Peoples R China
[2] Shanghai Fengxian Dist Cent Hosp, Dept Neurosurg, Shanghai 201400, Peoples R China
[3] Jilin Univ, Sch Publ Hlth, Changchun 130021, Peoples R China
关键词
Forkhead box protein C2 (FOXC2); angiogenesis; cell proliferation; ovarian cancer; EPITHELIAL-MESENCHYMAL TRANSITION; FORKHEAD TRANSCRIPTION FACTORS; ANGIOGENESIS; EXPRESSION; METASTASIS; CARCINOMA; LYMPHANGIOGENESIS; PROMOTES; INVASION;
D O I
10.3978/j.issn.2218-676X.2015.10.04
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: Forkhead box protein C2 (FOXC2) is an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor and involved in several cancer developments. In this study, we aimed to investigate the role of FOXC2 in ovarian cancer. Methods: A total of 13 ovarian tissues (11 cases of epithelial ovarian cancer, one case of borderline serous cystadenoma and one case of normal ovarian tissue) were collected and tissue specimens were stained to detect the expression and location of FOXC2 in ovarian cancer by immunohistochemistry. Then shRNAs targeting FOXC2 mRNA were designed to construct FOXC2-shRNA-expressing lentiviral vector (LV-FOXC2). Real-time RT-PCR and western blot assays were conducted to examine the interference effects of shRNA to FOXC2. Additionally, cell counting kit-8 (CCK8) was performed to detect cell proliferation of ID8 cells (mouse ovarian cancer cell) infected with FOXC2-shRNA-lentivirus. Results: No expression of FOXC2 was found in normal ovarian tissue and a large number of FOXC2 positive cells were found in borderline serous cystadenoma and ovarian cancer tissues including vascular endothelial cells, and FOXC2 was mainly seen in nucleus of ovarian cancer cells. Interference lentivirus of FOXC2 was successfully constructed with the virus titers of 1.2x10(8)similar to 1.3x10(8) Tu/mL, which significantly decreased the mRNA and protein expression levels of FOXC2 (P<0.05). Furthermore, cell proliferation was significantly suppressed with a time-dependent effect in ID8 cells infected with FOXC2-shRNA-lentivirus. Conclusions: This study might help to understand the molecular mechanism of FOXC2 in ovarian cancer and provide theoretical foundation for the development of diagnosis and treatment for ovarian cancer by targeting FOXC2.
引用
收藏
页码:453 / 459
页数:7
相关论文
共 50 条
  • [31] Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2
    Sarkar, Phoebe L.
    Lee, Wendy
    Williams, Elizabeth D.
    Lubik, Amy A.
    Stylianou, Nataly
    Shokoohmand, Ali
    Lehman, Melanie L.
    Hollier, Brett G.
    Gunter, Jennifer H.
    Nelson, Colleen C.
    FRONTIERS IN ENDOCRINOLOGY, 2019, 10
  • [32] Silencing of type Iγ phosphatidylinositol phosphate kinase suppresses ovarian cancer cell proliferation, migration and invasion
    Cao, Siyu
    Chen, Chunhua
    Xue, Junli
    Huang, Yan
    Yang, Xiaofeng
    Ling, Kun
    ONCOLOGY REPORTS, 2017, 38 (01) : 253 - 262
  • [33] Sam68 is Overexpressed in Epithelial Ovarian Cancer and Promotes Tumor Cell Proliferation
    Dong, Lijuan
    Che, Hailuo
    Li, Mingmei
    Li, Xuepeng
    MEDICAL SCIENCE MONITOR, 2016, 22 : 3248 - 3256
  • [34] Foxc1 and Foxc2 are necessary to maintain glomerular podocytes
    Motojima, Masaru
    Kume, Tsutomu
    Matsusaka, Taiji
    EXPERIMENTAL CELL RESEARCH, 2017, 352 (02) : 265 - 272
  • [35] FOXC2Disease Mutations Identified in Lymphedema Distichiasis Patients Impair Transcriptional Activity and Cell Proliferation
    Tavian, Daniela
    Missaglia, Sara
    Michelini, Sandro
    Maltese, Paolo Enrico
    Manara, Elena
    Mordente, Alvaro
    Bertelli, Matteo
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (14) : 1 - 18
  • [36] Akt-Activated Endothelium Increases Cancer Cell Proliferation and Resistance to Treatment in Ovarian Cancer Cell Organoids
    Hoarau-Vechot, Jessica
    Blot-Dupin, Morgane
    Pauly, Lea
    Touboul, Cyril
    Rafii, Shahin
    Rafii, Arash
    Pasquier, Jennifer
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (22)
  • [37] FOXC2 promotes epithelial-mesenchymal transition and cisplatin resistance of non-small cell lung cancer cells
    He, Yuwen
    Xie, Hui
    Yu, Pengjiu
    Jiang, Shunjun
    Wei, Li
    CANCER CHEMOTHERAPY AND PHARMACOLOGY, 2018, 82 (06) : 1049 - 1059
  • [38] RNA-seq Analysis of Wild-Type vs. FOXC2-Deficient Melanoma Cells Reveals a Role for the FOXC2 Transcription Factor in the Regulation of Multiple Oncogenic Pathways
    Hargadon, Kristian M.
    Williams, Corey J.
    FRONTIERS IN ONCOLOGY, 2020, 10
  • [39] FOXC2/ADAM12-dependent radiosensitivity of head and neck squamous cell carcinoma cells
    Jing, Zhibin
    Guo, Sitong
    Li, Yao
    Liang, Zheng
    HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK, 2022, 44 (01): : 212 - 225
  • [40] RETRACTED: FOXC2 promotes colorectal cancer proliferation through inhibition of FOX03a and activation of MAPK and AKT signaling pathways (Retracted Article)
    Cui, Yan-Mei
    Jiang, Dan
    Zhang, Shi-Hong
    Wu, Ping
    Ye, Ya-Ping
    Chen, Cui-Min
    Tang, Na
    Liang, Li
    Li, Ting-Ting
    Qi, Lu
    Wang, Shu-Yang
    Jiao, Hong-Li
    Lin, Jie
    Ding, Yan-Qing
    Liao, Wen-Ting
    CANCER LETTERS, 2014, 353 (01) : 87 - 94