Validation of three analytical methods for quantification of acetaminophen by UV spectrophotometry

Introduction: Acetaminophen is a non-steroidal analgesic and antipyretic, whose mechanism is based on the inhibition of the cyclooxygenase enzyme responsible for the appearance of pain and inflammation. In Mexico, it is one of the drugs widely used for its effectiveness, which is why the objective of this study was to implement a method of chemical validation by ultraviolet spectrophotometry that allows the quantification of this active principle. Method: We worked with 4 dissolution media (HCl: 0.1N MeOH, 0.1M HCl, Methanol: Water in a ratio of 15:85 p / v and 0.2M phosphate buffer). All four media underwent analytical validation, measuring linear regression significance, precision, sample stability, and sensitivity. Results: The 0.1M HCl, MeOH: H2O (15:85 v / v) and Phosphate Buffer media comply with the significance of the intercept, linearity of the method, as well as the parameters of precision, stability, and with the limits of detection (DL) and the limit of quantification (QL). Discarding the HCl: 0.1N MeOH medium for not meeting the linearity parameters. Conclusions: Of the fourth means evaluated, three of them (0.1M HCl, Methanol: water, and phosphate buffer) can be used as alternatives in the quantification of this drug.