Plasma Proteomics Identify Biomarkers and Pathogenesis of COVID-19

被引：241

作者：

Shu, Ting ^{[1
,2
,3
,4
]}

Ning, Wanshan ^{[5
]}

Wu, Di ^{[3
,6
,7
]}

Xu, Jiqian ^{[8
]}

Han, Qiangqiang ^{[9
]}

Huang, Muhan ^{[3
,6
,7
]}

Zou, Xiaojing ^{[8
]}

Yang, Qingyu ^{[1
,2
,3
,4
]}

Yuan, Yang ^{[1
,2
,4
]}

Bie, Yuanyuan ^{[3
,10
]}

Pan, Shangwen ^{[8
]}

Mu, Jingfang ^{[3
,6
,7
]}

Han, Yang ^{[1
,2
,3
,4
]}

Yang, Xiaobo ^{[8
]}

Zhou, Hong ^{[1
,2
,4
]}

Li, Ruiting ^{[8
]}

Ren, Yujie ^{[3
,6
,7
]}

Chen, Xi ^{[9
]}

Yao, Shanglong ^{[11
,12
]}

Qiu, Yang ^{[3
,4
,6
,7
,10
]}

Zhang, Ding-Yu ^{[1
,2
,4
,13
]}

Xue, Yu ^{[5
]}

Shang, You ^{[8
,11
,12
,13
]}

Zhou, Xi ^{[3
,4
,6
,7
,10
]}

机构：

[1] Wuhan Jinyintan Hosp, Wuhan Inst Virol, Joint Lab Infect Dis & Hlth, Wuhan 430023, Hubei, Peoples R China

[2] Wuhan Jinyintan Hosp, Wuhan 430023, Hubei, Peoples R China

[3] Chinese Acad Sci, Ctr Biosafety Megasci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China

[4] Wuhan Jinyintan Hosp, Ctr Translat Med, Wuhan 430023, Hubei, Peoples R China

[5] Huazhong Univ Sci & Technol HUST, Coll Life Sci & Technol, Ctr Artificial Intelligence Biol, Hubei Bioinformat & Mol Imaging Key Lab,MOE Key L, Wuhan 430074, Hubei, Peoples R China

[6] Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Infect Dis & Hlth, Wuhan 430023, Hubei, Peoples R China

[7] Chinese Acad Sci, Wuhan Jinyintan Hosp, Wuhan 430023, Hubei, Peoples R China

[8] HUST, Tongji Med Coll, Union Hosp, Dept Crit Care Med, Wuhan 430030, Hubei, Peoples R China

[9] SpecAlly Life Technol Co Ltd, Wuhan 430075, Hubei, Peoples R China

[10] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[11] HUST, Tongji Med Coll, Union Hosp, Inst Anesthesiol & Crit Care Med, Wuhan 430030, Hubei, Peoples R China

[12] Clin Res Ctr Anesthesiol Hubei Prov, Wuhan 430030, Peoples R China

[13] Chinese Acad Sci, Ctr Biosafety Megasci, Wuhan 430071, Hubei, Peoples R China

来源：

IMMUNITY | 2020年 / 53卷 / 05期

基金：

中国国家自然科学基金;

关键词：

L-PLASTIN; FETUIN-B; PROTEIN; DISEASE; FRACTIONATION; MICE;

D O I：

10.1016/j.immuni.2020.10.008

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The coronavirus disease 2019 (COVID-19) pandemic is a global public health crisis. However, little is known about the pathogenesis and biomarkers of COVID-19. Here, we profiled host responses to COVID-19 by performing plasma proteomics of a cohort of COVID-19 patients, including non-survivors and survivors recovered from mild or severe symptoms, and uncovered numerous COVID-19-associated alterations of plasma proteins. We developed a machine-learning-based pipeline to identify 11 proteins as biomarkers and a set of biomarker combinations, which were validated by an independent cohort and accurately distinguished and predicted COVID-19 outcomes. Some of the biomarkers were further validated by enzyme-linked immunosorbent assay (ELISA) using a larger cohort. These markedly altered proteins, including the biomarkers, mediate pathophysiological pathways, such as immune or inflammatory responses, platelet degranulation and coagulation, and metabolism, that likely contribute to the pathogenesis. Our findings provide valuable knowledge about COVID-19 biomarkers and shed light on the pathogenesis and potential therapeutic targets of COVID-19.

引用

页码：1108 / +

页数：20

共 62 条

[1] Use of a Surface Plasmon Resonance Method To Investigate Antibiotic and Plasma Protein Interactions [J].

Baneres-Roquet, Francoise ;

Gualtieri, Maxime ;

Villain-Guillot, Philippe ;

Pugniere, Martine ;

Leonetti, Jean-Paul .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (04) :1528-1531

[2] Untitled [J].

Bateman, Alex .

NUCLEIC ACIDS RESEARCH, 2006, 34 :D1-D1

[3] Off-Line High-pH Reversed-Phase Fractionation for In-Depth Phosphoproteomics [J].

Batth, Tanveer S. ;

Francavilla, Chiara ;

Olsen, Jesper V. .

JOURNAL OF PROTEOME RESEARCH, 2014, 13 (12) :6176-6186

[4] Pharmacokinetics, tolerability, and preliminary efficacy of paquinimod (ABR-215757), a new quinoline-3-carboxamide derivative: Studies in lupus-prone mice and a multicenter, randomized, double-blind, placebo-controlled, repeat-dose, dose-ranging study in patients with systemic lupus erythematosus [J].

Bengtsson, Anders A. ;

Sturfelt, Gunnar ;

Lood, Christian ;

Ronnblom, Lars ;

van Vollenhoven, Ronald F. ;

Axelsson, Bengt ;

Sparre, Birgitta ;

Tuvesson, Helen ;

Ohman, Marie Wallen ;

Leanderson, Tomas .

ARTHRITIS AND RHEUMATISM, 2012, 64 (05) :1579-1588

[5] Identification of Human S100A9 as a Novel Target for Treatment of Autoimmune Disease via Binding to Quinoline-3-Carboxamides [J].

Bjork, Per ;

Bjork, Anders ;

Vogl, Thomas ;

Stenstrom, Martin ;

Liberg, David ;

Olsson, Anders ;

Roth, Johannes ;

Ivars, Fredrik ;

Leanderson, Tomas .

PLOS BIOLOGY, 2009, 7 (04) :800-812

[6] mentha: a resource for browsing integrated protein-interaction networks [J].

Calderone, Alberto ;

Castagnoli, Luisa ;

Cesareni, Gianni .

NATURE METHODS, 2013, 10 (08) :690-691

[7] The Gene Ontology Resource: 20 years and still GOing strong [J].

Carbon, S. ;

Douglass, E. ;

Dunn, N. ;

Good, B. ;

Harris, N. L. ;

Lewis, S. E. ;

Mungall, C. J. ;

Basu, S. ;

Chisholm, R. L. ;

Dodson, R. J. ;

Hartline, E. ;

Fey, P. ;

Thomas, P. D. ;

Albou, L. P. ;

Ebert, D. ;

Kesling, M. J. ;

Mi, H. ;

Muruganujian, A. ;

Huang, X. ;

Poudel, S. ;

Mushayahama, T. ;

Hu, J. C. ;

LaBonte, S. A. ;

Siegele, D. A. ;

Antonazzo, G. ;

Attrill, H. ;

Brown, N. H. ;

Fexova, S. ;

Garapati, P. ;

Jones, T. E. M. ;

Marygold, S. J. ;

Millburn, G. H. ;

Rey, A. J. ;

Trovisco, V. ;

dos Santos, G. ;

Emmert, D. B. ;

Falls, K. ;

Zhou, P. ;

Goodman, J. L. ;

Strelets, V. B. ;

Thurmond, J. ;

Courtot, M. ;

Osumi-Sutherland, D. ;

Parkinson, H. ;

Roncaglia, P. ;

Acencio, M. L. ;

Kuiper, M. ;

Laegreid, A. ;

Logie, C. ;

Lovering, R. C. .

NUCLEIC ACIDS RESEARCH, 2019, 47 (D1) :D330-D338

[8] The Immune Functions of α1 Acid Glycoprotein [J].

Ceciliani, Fabrizio ;

Lecchi, Cristina .

CURRENT PROTEIN & PEPTIDE SCIENCE, 2019, 20 (06) :505-524

[9] Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study [J].

Chen, Nanshan ;

Zhou, Min ;

Dong, Xuan ;

Qu, Jieming ;

Gong, Fengyun ;

Han, Yang ;

Qiu, Yang ;

Wang, Jingli ;

Liu, Ying ;

Wei, Yuan ;

Xia, Jia'an ;

Yu, Ting ;

Zhang, Xinxin ;

Zhang, Li .

LANCET, 2020, 395 (10223) :507-513

[10] Downregulation of Fetuin-B and Zinc-α2-glycoprotein is Linked to Impaired Fatty Acid Metabolism in Liver Cells [J].

Choi, Jung-Won ;

Liu, Hao ;

Mukherjee, Rajib ;

Yun, Jong Won .

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2012, 30 (02) :295-306

← 1 2 3 4 5 6 7 →