A photometric method for organophosphorus pesticide detection based on microfluidic chip

Water pesticide pollution is one of the important environmental issues which need to be urgently solved, but organophosphorus pesticide detection in water environment often relies on traditional laboratory techniques which have poor timeliness and low degree of automation. The paper proposes a photometric detection based on microfluidic chip. We designed a special microfluidic chip according to the characteristics of water pollution pesticide detection and created an automation system platform including sample introducing, mixing, reacting and detecting on the basis of organic phosphorus pesticide hydrolysis reaction. The working parameters of the detection system were optimized under microscale conditions. Experimental results showed that the designed system parallels traditional chromatography in detecting the organic phosphorus pesticide content in seawater, with a correlation coefficient of 0.963. At the same time, reagent dosage of the designed system is one-thousandth of that in traditional chromatography, and the degree of automation is greatly improved.