Improvement of efficiency of genetic transformation for Dunaliella salina by glass beads method

被引：78

作者：

Feng, Shuying ^{[1
]}

Xue, Lexun ^{[1
]}

Liu, Hongtao ^{[1
]}

Lu, Pengju ^{[1
]}

机构：

[1] Zhengzhou Univ, Coll Med, Affiliated Hosp 1, Cell Biol Lab, Zhengzhou 450052, Henan, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2009年 / 36卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Dunaliella salina; GUS gene; Transformation; Glass beads; CHLAMYDOMONAS-REINHARDTII; HALOTOLERANT ALGAE; STABLE EXPRESSION; FOREIGN GENES; GREEN-ALGA; ELECTROPORATION; BIOTECHNOLOGY; SYSTEM;

D O I：

10.1007/s11033-008-9333-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dunaliella salina has been exploited as a new type of bioreactor due to its unique advantages. However, this bioreactor application was restricted for absence of a high-efficiency and stable transformation method at present. In the present study, the cells of D. salina were transformed by glass beads. The results of histochemical staining revealed that the GUS gene was successfully expressed in the positive transformants, and PCR and PCR-Southern blot analysis further demonstrated that the bar gene was integrated into the D. salina genome. Moreover, the three transformation methods, including glass beads, bombardment particle and electroporation, were compared for screening a high-efficiency transformation method for gene engineering of D. salina. The results showed that transformation efficiency of the glass beads was the highest, approximately 10(2) transformants/mu g DNA. It is concluded that the established glass beads method has been demonstrated to be an optimal transformation way for D. salina.

引用

页码：1433 / 1439

页数：7

共 22 条

[1] THE OSMOTIC COMPONENTS OF HALOTOLERANT ALGAE
AVRON, M
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 1986, 11 (01) : 5 - 6
[2] GLYCEROL PRODUCTION BY DUNALIELLA
BENAMOTZ, A
SUSSMAN, I
AVRON, M
[J]. EXPERIENTIA, 1982, 38 (01): : 49 - 52
[3] THE BIOTECHNOLOGY OF CULTIVATING THE HALOTOLERANT ALGA DUNALIELLA
BENAMOTZ, A
AVRON, M
[J]. TRENDS IN BIOTECHNOLOGY, 1990, 8 (05) : 121 - 126
[4] ACCUMULATION OF METABOLITES BY HALOTOLERANT ALGAE AND ITS INDUSTRIAL POTENTIAL
BENAMOTZ, A
AVRON, M
[J]. ANNUAL REVIEW OF MICROBIOLOGY, 1983, 37 : 95 - 119
[5] THE BIOTECHNOLOGY OF CULTIVATING DUNALIELLA FOR PRODUCTION OF BETA-CAROTENE RICH ALGAE
BENAMOTZ, A
SHAISH, A
AVRON, M
[J]. BIORESOURCE TECHNOLOGY, 1991, 38 (2-3) : 233 - 235
[6] CHLOROPLAST TRANSFORMATION IN CHLAMYDOMONAS WITH HIGH-VELOCITY MICROPROJECTILES
BOYNTON, JE
GILLHAM, NW
HARRIS, EH
HOSLER, JP
JOHNSON, AM
JONES, AR
RANDOLPHANDERSON, BL
ROBERTSON, D
KLEIN, TM
SHARK, KB
SANFORD, JC
[J]. SCIENCE, 1988, 240 (4858) : 1534 - 1538
[7] INTRODUCTION OF EXOGENOUS DNA INTO CHLAMYDOMONAS-REINHARDTII BY ELECTROPORATION
BROWN, LE
SPRECHER, SL
KELLER, LR
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (04) : 2328 - 2332
[8] Highly efficient expression of rabbit neutrophil peptide-1 gene in Chlorella ellipsoidea cells
Chen, Y
Wang, YQ
Sun, YR
Zhang, LM
Li, WB
[J]. CURRENT GENETICS, 2001, 39 (5-6) : 365 - 370
[9] COSTANZO MC, 1988, GENETICS, V120, P667
[10] DUNAHAY TG, 1993, BIOTECHNIQUES, V15, P452

← 1 2 3 →