共 36 条
Examination of seroprevalence of coronavirus HKU1 infection with S protein-based ELISA and neutralization assay against viral spike pseudotyped virus
被引:70
作者:
Chan, C. M.
[1
,2
,3
]
Tse, Herman
[1
,2
,3
]
Wong, S. S. Y.
[1
,2
,3
]
Woo, P. C. Y.
[1
,2
,3
]
Lau, S. K. P.
[1
,2
,3
]
Chen, L.
[1
,2
,3
]
Zheng, B. J.
[1
,2
,3
]
Huang, J. D.
[1
,2
,3
]
Yuen, K. Y.
[1
,2
,3
]
机构:
[1] Dept Microbiol, State Key Lab Emerging Infect Dis, Hong Kong, Hong Kong, Peoples R China
[2] Univ Hong Kong, Res Ctr Infect & Immunol, Hong Kong, Hong Kong, Peoples R China
[3] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Beijing 100864, Peoples R China
关键词:
Human coronavirus HKU1;
Spike;
Serorepidemiology;
Neutralization antibody;
SARS-COV;
PROTECTIVE IMMUNITY;
VACCINIA VIRUS;
HONG-KONG;
CHILDREN;
ANTIBODY;
PNEUMONIA;
POLYPEPTIDE;
SEQUENCE;
RECEPTOR;
D O I:
10.1016/j.jcv.2009.02.011
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
Background: Human coronavirus HKU1 (HCoV-HKU1) is a recently identified coronavirus with a global distribution and known to cause mainly respiratory infections. Objectives: To investigate the seroepidemiology of HKU1 infections in our local population. Study design: An ELISA-based IgG antibody detection assay using recombinant HCoV-HKU1 nucleocapsid and spike (S) proteins (genotype A) were developed for the diagnosis of CoV-HKU1 infections, Additionally, a neutralization antibody assay using the HCoV-HKU1 pseudotyped virus was developed to detect the presence of neutralizing antibodies in serum with antibody positivity in an S protein-based ELISA. Results: Results of the recombinant S protein-based ELISA were validated with Western blot, immunofluorescence analysis and flow cytometry. The coupled results demonstrated good correlation with Spearmen's coefficient of 0.94. Seroepidemiological study in a local hospital-based setting using this newly developed ELISA showed steadily increasing HCoV-HKU1 seroprevalence in childhood and early adulthood, front 0% in the age group of <10 years old to a plateau of 21.6% in the age group of 31-40 years old. Conclusions: Our study demonstrated the usefulness of the S-based ELISA which could be applied to future epidemiological studies of HCoV-HKU1 in other localities. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:54 / 60
页数:7
相关论文