Examination of seroprevalence of coronavirus HKU1 infection with S protein-based ELISA and neutralization assay against viral spike pseudotyped virus

被引:70
作者
Chan, C. M. [1 ,2 ,3 ]
Tse, Herman [1 ,2 ,3 ]
Wong, S. S. Y. [1 ,2 ,3 ]
Woo, P. C. Y. [1 ,2 ,3 ]
Lau, S. K. P. [1 ,2 ,3 ]
Chen, L. [1 ,2 ,3 ]
Zheng, B. J. [1 ,2 ,3 ]
Huang, J. D. [1 ,2 ,3 ]
Yuen, K. Y. [1 ,2 ,3 ]
机构
[1] Dept Microbiol, State Key Lab Emerging Infect Dis, Hong Kong, Hong Kong, Peoples R China
[2] Univ Hong Kong, Res Ctr Infect & Immunol, Hong Kong, Hong Kong, Peoples R China
[3] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Beijing 100864, Peoples R China
关键词
Human coronavirus HKU1; Spike; Serorepidemiology; Neutralization antibody; SARS-COV; PROTECTIVE IMMUNITY; VACCINIA VIRUS; HONG-KONG; CHILDREN; ANTIBODY; PNEUMONIA; POLYPEPTIDE; SEQUENCE; RECEPTOR;
D O I
10.1016/j.jcv.2009.02.011
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: Human coronavirus HKU1 (HCoV-HKU1) is a recently identified coronavirus with a global distribution and known to cause mainly respiratory infections. Objectives: To investigate the seroepidemiology of HKU1 infections in our local population. Study design: An ELISA-based IgG antibody detection assay using recombinant HCoV-HKU1 nucleocapsid and spike (S) proteins (genotype A) were developed for the diagnosis of CoV-HKU1 infections, Additionally, a neutralization antibody assay using the HCoV-HKU1 pseudotyped virus was developed to detect the presence of neutralizing antibodies in serum with antibody positivity in an S protein-based ELISA. Results: Results of the recombinant S protein-based ELISA were validated with Western blot, immunofluorescence analysis and flow cytometry. The coupled results demonstrated good correlation with Spearmen's coefficient of 0.94. Seroepidemiological study in a local hospital-based setting using this newly developed ELISA showed steadily increasing HCoV-HKU1 seroprevalence in childhood and early adulthood, front 0% in the age group of <10 years old to a plateau of 21.6% in the age group of 31-40 years old. Conclusions: Our study demonstrated the usefulness of the S-based ELISA which could be applied to future epidemiological studies of HCoV-HKU1 in other localities. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:54 / 60
页数:7
相关论文
共 36 条
[1]   Neutralizing antibody and protective immunity to SARS coronavirus infection of mice induced by a soluble recombinant polypeptide containing an N-terminal segment of the spike glycoprotein [J].
Bisht, H ;
Roberts, A ;
Vogel, L ;
Subbarao, K ;
Moss, B .
VIROLOGY, 2005, 334 (02) :160-165
[2]   Severe acute respiratory syndrome coronavirus spike protein expressed by attenuated vaccinia virus protectively immunizes mice [J].
Bisht, H ;
Roberts, A ;
Vogel, L ;
Bukreyev, A ;
Collins, PL ;
Murphy, BR ;
Subbarao, K ;
Moss, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (17) :6641-6646
[3]   Spike protein assembly into the coronavirion: Exploring the limits of its sequence requirements [J].
Bosch, BJ ;
de Haan, CAM ;
Smits, SL ;
Rottler, PJM .
VIROLOGY, 2005, 334 (02) :306-318
[4]   Coronavirus HKU1 in an Italian pre-term infant with bronchiolitis [J].
Bosis, Samantha ;
Esposito, Susanna ;
Niesters, Hubert G. M. ;
Tremolati, Elena ;
Pas, Susan ;
Principi, Nicola ;
Osterhaus, Albert D. M. E. .
JOURNAL OF CLINICAL VIROLOGY, 2007, 38 (03) :251-253
[5]   RETRACTED: Spike Protein, S, of Human Coronavirus HKU1: Role in Viral Life Cycle and Application in Antibody Detection (Retracted Article) [J].
Chan, Che-Man ;
Woo, Patrick C. Y. ;
Lau, Susanna K. P. ;
Tse, Herman ;
Chen, Hong-Lin ;
Li, Feng ;
Zheng, Bo-Jian ;
Chen, Ling ;
Huang, Jian-Dong ;
Yuen, Kwok-Yung .
EXPERIMENTAL BIOLOGY AND MEDICINE, 2008, 233 (12) :1527-1536
[6]   Efficacy and safety analyses of a recombinant human immunodeficiency virus type 1 derived vector system [J].
Chang, LJ ;
Urlacher, V ;
Iwakuma, T ;
Cui, Y ;
Zucali, J .
GENE THERAPY, 1999, 6 (05) :715-728
[7]   Detection of viruses identified recently in children with acute wheezing [J].
Chung, Ju-Young ;
Han, Tae Hee ;
Kim, Sang Woo ;
Kim, Chang Keun ;
Hwang, Eung-Soo .
JOURNAL OF MEDICAL VIROLOGY, 2007, 79 (08) :1238-1243
[8]   Receptor-binding domain of SARS-CoV spike protein induces long-term protective immunity in an animal model [J].
Du, Lanying ;
Zhao, Guangyu ;
He, Yuxian ;
Guo, Yan ;
Zheng, Bo-Jian ;
Jiang, Shibo ;
Zhou, Yusen .
VACCINE, 2007, 25 (15) :2832-2838
[9]   Coronavirus HKU1 infection in the United States [J].
Esper, F ;
Weibel, C ;
Ferguson, D ;
Landry, ML ;
Kahn, JS .
EMERGING INFECTIOUS DISEASES, 2006, 12 (05) :775-779
[10]   Impact of human coronavirus infections in otherwise healthy children who attended an emergency department [J].
Esposito, Susanna ;
Bosis, Samantha ;
Niesters, Hubert G. M. ;
Tremolati, Elena ;
Begliatti, Enrica ;
Rognoni, Alessandro ;
Tagliabue, Claudia ;
Principi, Nicola ;
Osterhaus, Albert D. M. E. .
JOURNAL OF MEDICAL VIROLOGY, 2006, 78 (12) :1609-1615