Doxycycline Enhances Survival and Self-Renewal of Human Pluripotent Stem Cells

被引：50

作者：

Chang, Mi-Yoon ^{[1
,2
]}

Rhee, Yong-Hee ^{[1
,2
,3
]}

Yi, Sang-Hoon ^{[1
,2
]}

Lee, Su-Jae ^{[4
]}

Kim, Rae-Kwon ^{[4
]}

Kim, Hyongbum ^{[2
,3
]}

Park, Chang-Hwan ^{[2
,3
]}

Lee, Sang-Hun ^{[1
,2
,3
]}

机构：

[1] Hanyang Univ, Coll Med, Dept Biochem & Mol Biol, Seoul 133791, South Korea

[2] Hanyang Univ, Hanyang Biomed Res Inst, Seoul 133791, South Korea

[3] Hanyang Univ, Grad Sch Biomed Sci & Engn, Seoul 133791, South Korea

[4] Hanyang Univ, Coll Nat Sci, Dept Chem, Seoul 133791, South Korea

来源：

STEM CELL REPORTS | 2014年 / 3卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

DOPAMINE NEURONS; IN-VITRO; MICROGLIAL ACTIVATION; NEURAL PRECURSORS; HUMAN BLASTOCYSTS; RAT MODEL; DIFFERENTIATION; MINOCYCLINE; DERIVATION; ISCHEMIA;

D O I：

10.1016/j.stemcr.2014.06.013

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

We here report that doxycycline, an antibacterial agent, exerts dramatic effects on human embryonic stem and induced pluripotent stem cells (hESC/iPSCs) survival and self-renewal. The survival-promoting effect was also manifest in cultures of neural stem cells (NSCs) derived from hESC/iPSCs. These doxycycline effects are not associated with its antibacterial action, but mediated by direct activation of a PI3K-AKT intracellular signal. These findings indicate doxycycline as a useful supplement for stem cell cultures, facilitating their growth and maintenance.

引用

页码：353 / 364

页数：12

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