Time-resolved infrared spectroscopy in the study of photosynthetic systems

被引:34
作者
Mezzetti, Alberto [1 ,2 ]
Leibl, Winfried [2 ]
机构
[1] UPMC Univ Paris 06, Sorbonne Univ, CNRS, UMR 7197,Lab Reactivite Surfaces, 4 Pl Jussieu, F-75005 Paris, France
[2] Univ Paris Saclay, Univ Paris Sud, I2BC, IBITECS,CEA,CNRS, F-91198 Gif Sur Yvette, France
关键词
FTIR difference spectroscopy; Step-scan FTIR; Vibrational spectroscopy; Rapid-scan FTIR; Infrared; Electron transfer; Proton transfer; Reaction centers; Ubiquinone; Light-harvesting systems; Bacterial reaction centers; Photosystem I; Photosystem II; FTIR DIFFERENCE SPECTROSCOPY; SPHAEROIDES REACTION CENTERS; CHLOROPHYLL-A-PROTEIN; D-CENTER-DOT; ELECTRON-TRANSFER; RHODOBACTER-SPHAEROIDES; PHOTOSYSTEM-II; PROTON UPTAKE; HYBRID HARD; VIBRATIONAL PROPERTIES;
D O I
10.1007/s11120-016-0305-3
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Time-resolved (TR) infrared (IR) spectroscopy in the nanosecond to second timescale has been extensively used, in the last 30 years, in the study of photosynthetic systems. Interesting results have also been obtained at lower time resolution (minutes or even hours). In this review, we first describe the used techniques-dispersive IR, laser diode IR, rapid-scan Fourier transform (FT)IR, step-scan FTIR-underlying the advantages and disadvantages of each of them. Then, the main TR-IR results obtained so far in the investigation of photosynthetic reactions (in reaction centers, in light-harvesting systems, but also in entire membranes or even in living organisms) are presented. Finally, after the general conclusions, the perspectives in the field of TR-IR applied to photosynthesis are described.
引用
收藏
页码:121 / 144
页数:24
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