Molecules involve in the self-protection of neurons against glucose-oxygen-serum deprivation (GOSD)-induced cell damage

被引：10

作者：

Wang, Chen-Hsuan ^{[1
,2
]}

Lee, Wen-Jane ^{[3
]}

Ghanta, Vithal K. ^{[4
]}

Wang, Wei-Ti ^{[1
]}

Cheng, Shu-Yun ^{[1
]}

Hsueh, Chi-Mei ^{[1
]}

机构：

[1] Natl Chung Hsing Univ, Dept Life Sci, Taichung 40227, Taiwan

[2] Natl Taiwan Sport Univ, Dept Adapted Phys Educ, Tao Yuan 33301, Taiwan

[3] Taichung Vet Gen Hosp, Dept Educ & Res, Taichung 407, Taiwan

[4] Univ Alabama Birmingham, Dept Biol, Birmingham, AL 35294 USA

来源：

BRAIN RESEARCH BULLETIN | 2009年 / 79卷 / 3-4期

关键词：

GOSD; Neurotrophic factors; Inflammatory factors; Leptin; Superoxide; NO; ACTIVATED PROTEIN-KINASE; NITRIC-OXIDE; CYCLOOXYGENASE-2; EXPRESSION; INFLAMMATORY RESPONSE; OXIDATIVE STRESS; CORTICAL-NEURONS; UP-REGULATION; BRAIN-CELLS; LEPTIN; NEUROPROTECTION;

D O I：

10.1016/j.brainresbull.2009.02.006

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Molecules involved in self-protection of neurons against glucose/oxygen/serum deprivation (GOSD) were investigated. Trypan blue dye exclusion assay, Western blotting, ELISA, cytokine antibody array and chemical blocking assay were applied in the study. Results showed that early induction (at 6 h of GOSD) of cyclooxygenase-2 (COX-2), leptin, transforming growth factor-beta 1 (TGF-beta 1), glial-cell-line-derived neurotrophic factor (GDNF) and neurotrophin-3 (NT-3) all played a compensatory role in the protection of neurons against GOSD. Decline of these molecules and peroxisome proliferators-activated receptor (PPAR)-gamma and -alpha since 12 h of GOSD may lead to an irreversible neuronal death. Nitric oxide (NO) and superoxide at low concentrations were neuroprotective whereas at high concentrations were detrimental to neurons. Accumulation of NO and superoxide at late stage of GOSD should therefore be prevented. The study provided a useful platform for screening of potential anti-ischemic drugs and also explained why GOSD neuron derived conditioned medium (NCM) only exerted a time-restricted neuroprotection. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：169 / 176

页数：8

共 35 条

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