Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil

被引：3

作者：

Rabahi, Marcelo Fouad ^{[1
]}

Conceicao, Emilyn Costa ^{[2
,3
]}

de Paiva, Luisa Oliveira ^{[1
]}

Muniz Lemes Souto, Marcos Vinicius ^{[1
]}

Sisco, Maria Carolina ^{[2
,4
]}

de Waard, Jacobus ^{[5
]}

de Souza Caldas, Paulo Cesar ^{[6
]}

Fandinho, Fatima ^{[6
]}

Ramos, Jesus Pais ^{[6
]}

de Carvalho, Luciana Distasio ^{[6
]}

Dias Campos, Carlos Eduardo ^{[6
]}

Batista Lima, Karla Valeria ^{[7
,12
,13
]}

da Silva, Sandro Patroca ^{[7
]}

Sharma, Abhinav ^{[8
]}

Robledo, Jaime ^{[9
]}

Hurtado Paez, Uriel Alonso ^{[9
]}

Duarte, Rafael Silva ^{[4
]}

da Silva, Marlei Gomes ^{[4
]}

Gomes, Lia Lima ^{[2
]}

Goncalves Vasconcellos, Sidra Ezidio ^{[2
]}

Uwezeye, Cecile ^{[10
]}

de Jong, Bouke C. ^{[10
]}

Junqueira-Kipnis, Ana Paula ^{[11
]}

Suffys, Philip Noel ^{[2
]}

机构：

[1] Univ Fed Goias, Hosp Clin, Setor Leste Univ, Primeira Ave S-N, BR-74605020 Goiania, Go, Brazil

[2] Fiocruz MS, Inst Oswaldo Cruz, Lab Biol Mol Aplicada Micobacterias, Ave Brasil 4365, BR-21040360 Rio De Janeiro, RJ, Brazil

[3] Fundacao Oswaldo Cruz, Inst Nacl Infectol Evandro Chagas, Programa Posgrad Pesquisa Clin & Doencas Infeccio, Ave Brasil 4365, BR-21040360 Rio De Janeiro, RJ, Brazil

[4] Univ Fed Rio de Janeiro, Inst Microbiol Prof Paulo de Goes, Ilha Cidade Univ S-N Bl 1 Ss, BR-21941970 Rio De Janeiro, RJ, Brazil

[5] Univ Amer, Fac Ciencias Salud, Hlth Res Grp 1, Quito, Ecuador

[6] Fiocruz MS, Ctr Referencia Prof Helio Fraga, Estr Curicica 2000, BR-22710560 Rio De Janeiro, RJ, Brazil

[7] Inst Evandro Chagas, Rodovia BR-316 KM7 S-N, BR-67030000 Ananindeua, PA, Brazil

[8] Int Inst Informat Technol Bangalore IIIT B, Dept Data Sci, Bangalore, Karnataka, India

[9] Corp Invest Biol CIB, Medellin, Colombia

[10] Inst Trop Med ITM, Mycobacteriol Unit, Nationalestr 155, B-2000 Antwerp, Belgium

[11] Univ Fed Goias, Inst Patol Trop & Saude Publ, Setor Univ, Primeira Ave, Goiania, Go, Brazil

[12] Univ Estado Para, Programa Posgrad Biol Parasitaria Amazonia, Trav Perebebui 2623, BR-66087670 Belem, PA, Brazil

[13] Inst Evandro Chagas, Trav Perebebui 2623, BR-66087670 Belem, PA, Brazil

来源：

INFECTION GENETICS AND EVOLUTION | 2020年 / 85卷

基金：

欧洲研究理事会;

关键词：

Mycobacterium tuberculosis var. africanum; Tuberculosis; Molecular epidemiology; Genome; Case report; Brazil; COMPLEX; EPIDEMIOLOGY; PATHOGEN; STRAINS;

D O I：

10.1016/j.meegid.2020.104550

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Human tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC), including Mycobacterium tuberculosis var. tuberculosis (MTB) and Mycobacterium tuberculosis var. africanum (MAF). While MTB is isolated worldwide, MAF is almost completely restricted to the African continent, and despite the historical proximity between Brazil and Africa during the slave trade, no case of TB being caused by MAF has been reported in Brazil to date. We hereby describe the first case of TB caused by MAF in Brazil comparing its genome against the published ones. A female patient who had never visited Africa presented with clinical symptoms typical of pulmonary TB. Based on 16S rRNA gene sequencing, the cultured isolate was identified as belonging to MTBC and partial sequence of the hsp65 gene was identical to that of MAF. This was confirmed by genotyping based on detection of Single Nucleotide Polymorphism (SNP), Region of Difference (RD) and spoligotyping. The isolate presented the Shared International Typing (SIT) 181. In the whole-genome comparison against MAF genomes available on published EMBL-EBI European Nucleotide Archive (ENA), the Brazilian genome (MAFBRA00707) was identified as belonging to Lineage 6 and clustered with isolates from The Gambia. This is the first report of the isolation of MAF from a patient from Brazil, without evidence of having any contact with an African index case.

引用

页数：7

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