Altered fecal short chain fatty acid composition in children with a history of Hirschsprung-associated enterocolitis

被引:60
作者
Demehri, Farokh R. [1 ]
Frykman, Philip K. [2 ,3 ,4 ]
Cheng, Zhi [2 ,3 ,4 ]
Ruan, Chunhai [5 ]
Wester, Tomas [6 ,7 ,8 ]
Nordenskjold, Agneta [6 ,7 ,8 ]
Kawaguchi, Akemi [9 ]
Hui, Thomas T. [10 ]
Granstrom, Anna L. [6 ,7 ,8 ]
Funari, Vince [11 ]
Teitelbaum, Daniel H. [1 ]
机构
[1] Univ Michigan, CS Mott Childrens Hosp, Div Pediat Surg, Ann Arbor, MI 48109 USA
[2] Cedars Sinai Med Ctr, Div Pediat Surg, Los Angeles, CA 90048 USA
[3] Cedars Sinai Med Ctr, Dept Surg, Los Angeles, CA 90048 USA
[4] Cedars Sinai Med Ctr, Dept Biomed Sci, Los Angeles, CA 90048 USA
[5] Univ Michigan, Michigan Comprehens Metabol Res Core, Ann Arbor, MI 48109 USA
[6] Karolinska Univ Hosp, Astrid Lindgrens Childrens Hosp, Dept Pediat Surg, Stockholm, Sweden
[7] Karolinska Inst, Dept Womens & Childrens Hlth, Stockholm, Sweden
[8] Karolinska Inst, Ctr Mol Med CMM, Stockholm, Sweden
[9] Childrens Hosp Los Angeles, Div Pediat Surg, Los Angeles, CA 90027 USA
[10] Childrens Hosp Oakland, Div Pediat Surg, Oakland, CA USA
[11] Cedars Sinai Med Ctr, Inst Med Genet, Genom Core Lab, Los Angeles, CA 90048 USA
关键词
Hirschsprung disease; Hirschsprung-associated enterocolitis; Aganglionosis; Pediatric; Short chain fatty acids; Microbiome; INTESTINAL DYSBIOSIS; BUTYRATE; INHIBITION; DISEASE; FERMENTATION; BACTERIA;
D O I
10.1016/j.jpedsurg.2015.10.012
中图分类号
R72 [儿科学];
学科分类号
100202 ;
摘要
Purpose: Children with Hirschsprung disease (HD) who have a history of enterocolitis (HAEC) have a shift in colonic microbiota, many of which are necessary for short chain fatty acid (SCFA) production. As SCFAs play a critical role in colonic mucosal preservation, we hypothesized that fecal SCFA composition is altered in children with HAEC. Methods: A multicenter study enrolled 18 HD children, abstracting for history of feeding, antibiotic/probiotic use, and enterocolitis symptoms. HAEC status was determined per Pastor et al. criteria (12). Fresh feces were collected for microbial community analysis via 16S sequencing as well as SCFA analysis by gas chromatography mass spectrometry. Results: Nine patients had a history of HAEC, and nine had never had HAEC. Fecal samples from HAEC children showed a 4-fold decline in total SCFA concentration vs. non-HAEC HD patients. We then compared the relative composition of individual SCFAs and found reduced acetate and increased butyrate in HAEC children. Finally, we measured relative abundance of SCFA-producing fecal microbiota. Interestingly, 10 of 12 butyrate producing genera as well as 3 of 4 acetate-producing genera demonstrated multi-fold expansion. Conclusion: Children with HAEC history have reduced fecal SCFAs and altered SCFA profile. These findings suggest a complex interplay between the colonic metabolome and changes in microbiota, which may influence the pathogenesis of HAEC. (C) 2016 Published by Elsevier Inc.
引用
收藏
页码:81 / 86
页数:6
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