Studies of interaction between two alkaloids and double helix DNA

被引：12

作者：

Sun, Yantao ^{[1
,2
]}

Peng, Tingting ^{[2
,3
]}

Zhao, Lei ^{[4
]}

Jiang, Dayu ^{[1
,2
]}

Cui, Yuncheng ^{[1
,2
]}

机构：

[1] Jilin Normal Univ, Minist Educ China, Key Lab Preparat & Applicat Environm Friendly Mat, Siping 136000, Peoples R China

[2] Jilin Normal Univ, Coll Chem, Siping 136000, Peoples R China

[3] Baicheng Normal Univ, Coll Chem, Baicheng 130032, Peoples R China

[4] Siping Inst Food & Drug Control, Siping 136000, Peoples R China

来源：

JOURNAL OF LUMINESCENCE | 2014年 / 156卷

关键词：

DNA; Alkaloids; Spectroscopic analysis; Viscosity measurement; Intercalation; NUCLEIC-ACID; BINDING; EVODIAMINE; MATRINE; MODE; TRIS(PHENANTHROLINE)RUTHENIUM(II); THERMODYNAMICS; PROLIFERATION; SANGUINARINE; FLUORESCENCE;

D O I：

10.1016/j.jlumin.2014.07.014

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

This article presents the study on the interaction of two alkaloids (matrine and evodiamine) and hs-DNA by absorption, fluorescence, circular dichroism (CD), DNA melting and viscosity experiments. The spectroscopic studies suggested that two alkaloids can bind to DNA through an intercalative mode. The viscosity measurement and thermal denaturation also indicated that two alkaloids can intercalate to DNA. The binding constants (K-A) and the number of binding sites (n) were determined. At the same time, some significant thermodynamic parameters of the binding of the alkaloids to DNA were obtained. Competitive binding studies revealed that alkaloids had an effect on ethidium bromide (EB) bound DNA. In addition, it was also proved that the fluorescence quenching was influenced by ionic strength. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：108 / 115

页数：8

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