Analysis of a plasmid encoding botulinum neurotoxin type G gene in Clostridium argentinense

被引：1

作者：

Sakaguchi, Yoshihiko ^{[1
]}

Uchiyama, Jumpei ^{[2
]}

Tak, Akira ^{[1
]}

Gotoh, Kazuyoshi ^{[3
]}

Sakaguchi, Masakiyo ^{[4
]}

Suzuki, Tomonori ^{[5
]}

Yamamoto, Yumiko ^{[3
]}

Hosomi, Koji ^{[6
,7
]}

Kohda, Tomoko ^{[8
]}

Mukamoto, Masafumi ^{[8
]}

Kozaki, Shunji ^{[8
]}

Hayashi, Shunji ^{[1
]}

Oguma, Keiji ^{[3
]}

机构：

[1] Kitasato Univ, Sch Med, Dept Microbiol, Sagamihara, Kanagawa, Japan

[2] Azabu Univ, Sch Vet Med, Lab Vet Microbiol 1, Sagamihara, Kanagawa, Japan

[3] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Bacteriol, Okayama, Okayama, Japan

[4] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Cell Biol, Okayama, Okayama, Japan

[5] Tokyo Univ Agr, Fac Life Sci, Dept Mol Microbiol, Tokyo, Japan

[6] Natl Inst Biomed Innovat Hlth & Nutr, Lab Vaccine Mat, Ctr Vaccine & Adjuvant Res, Ibaraki, Osaka, Japan

[7] Natl Inst Biomed Innovat Hlth & Nutr, Lab Gut Environm Syst, Ibaraki, Osaka, Japan

[8] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Dept Vet Sci, Osaka, Japan

来源：

ANAEROBE | 2020年 / 66卷

关键词：

Botulism; Clostridium argentinense; Botulinum neurotoxin type G; Anaerobe; Plasmid; TOXIN; PHAGE;

D O I：

10.1016/j.anaerobe.2020.102281

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Clostridium argentinense produces botulinum neurotoxin type G (BoNT/G). We sequenced and analyzed the plasmid harboring the bont/G gene, designated pCAG, in C. argentinense strain 2740. The pCAG consisted of 140,070 bp containing the bont/G gene cluster. Although this gene cluster showed high similarities in its DNA sequence and ORF arrangement to those of other bont gene clusters, the other regions of the plasmid did not. A phylogenetic study suggested that pCAG had a unique evolutionary history compared with other clostridial bont-harboring plasmids. This suggests that pCAG is possibly a novel type of plasmid expressing the bont/G gene in C. argentinense. (c) 2020 Elsevier Ltd. All rights reserved.

引用

页数：6