Modulation of miRNAs by Vitamin C in Human Bone Marrow Stromal Cells

被引：14

作者：

Kolhe, Ravindra ^{[1
]}

Mondal, Ashis K. ^{[1
]}

Pundkar, Chetan ^{[1
]}

Periyasamy-Thandavan, Sudharsan ^{[2
]}

Mendhe, Bharati ^{[2
]}

Hunter, Monte ^{[3
]}

Isales, Carlos M. ^{[3
,4
]}

Hill, William D. ^{[2
,4
]}

Hamrick, Mark W. ^{[2
,4
]}

Fulzele, Sadanand ^{[3
,4
]}

机构：

[1] Augusta Univ, Dept Pathol, Augusta, GA 30912 USA

[2] Augusta Univ, Cellular Biol & Anat, Augusta, GA 30912 USA

[3] Augusta Univ, Dept Orthopaed Surg, Augusta, GA 30912 USA

[4] Augusta Univ, Inst Regenerat & Reparat Med, Augusta, GA 30912 USA

来源：

NUTRIENTS | 2018年 / 10卷 / 02期

基金：

美国国家卫生研究院;

关键词：

bone marrow stromal cells; vitamin C; miRNA; MESENCHYMAL STEM-CELLS; POLYUNSATURATED FATTY-ACIDS; ASCORBIC-ACID; DNA DEMETHYLATION; TRANSPORTER SVCT2; DIFFERENTIATION; PROLIFERATION; MICRORNAS; PLURIPOTENCY; DEFICIENCY;

D O I：

10.3390/nu10020186

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

MicroRNAs (miRNAs) are small (18-25 nucleotides), noncoding RNAs that have been identified as potential regulators of bone marrow stromal cell (BMSC) proliferation, differentiation, and musculoskeletal development. Vitamin C is known to play a vital role in such types of biological processes through various different mechanisms by altering mRNA expression. We hypothesized that vitamin C mediates these biological processes partially through miRNA regulation. We performed global miRNA expression analysis on human BMSCs following vitamin C treatment using microarrays containing human precursor and mature miRNA probes. Bioinformatics analyses were performed on differentially expressed miRNAs to identify novel target genes and signaling pathways. Our bioinformatics analysis suggested that the miRNAs may regulate multiple stem cell-specific signaling pathways such as cell adhesion molecules (CAMs), fatty acid biosynthesis and hormone signaling pathways. Furthermore, our analysis predicted novel stem cell proliferation and differentiation gene targets. The findings of the present study demonstrate that vitamin C can have positive effects on BMSCs in part by regulating miRNA expression.

引用

页数：17