Enzymatic, outer membrane proteins and plasmid alterations of starved Vibrio parahaemolyticus and Vibrio alginolyticus cells in seawater

被引：24

作者：

Ben Abdallah, Fethi ^{[1
,2
]}

Kallel, Hela ^{[2
]}

Bakhrouf, Amina ^{[1
]}

机构：

[1] Fac Pharm, Lab Anal Traitement & Valorisat Polluants Environ, Monastir 5000, Tunisia

[2] Inst Pasteur, Unite Fermentat & Dev Vaccins Virol, Tunis 1002, Tunisia

来源：

ARCHIVES OF MICROBIOLOGY | 2009年 / 191卷 / 06期

关键词：

Vibrio; Seawater; Starvation; Enzymatic; Morphological; Outer membrane proteins; Plasmids; Alterations; ESCHERICHIA-COLI; PROTEOMIC ANALYSIS; PORIN EXPRESSION; YERSINIA-PESTIS; RESISTANCE; BACTERIA; GROWTH; LIPOPOLYSACCHARIDE; PERMEABILITY; TEMPERATURE;

D O I：

10.1007/s00203-009-0477-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The marine bacteria Vibrio parahaemolyticus and V. alginolyticus were incubated in seawater for 8 months to evaluate their adaptative responses to starvation. The starved cells showed an altered biochemical and enzymatic profiles, respectively, on Api 20E and Api ZYM systems and an evolution to the filterable minicells state capable to pass membrane pore size 0.45 mu m. Outer membrane proteins patterns of stressed bacteria were also altered. Indeed, these modifications were manifested by the appearance and/or disappearance of bands as well as in the level of expression of certain proteins. Plasmids profiles analysis showed that V. alginolyticus ATCC 33787 lost three plasmids, whereas other tested strains conserved their initial profiles.

引用

页码：493 / 500

页数：8

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[1] Phenotypic variations and molecular identification of Salmonella enterica serovar Typhimurium cells under starvation in seawater [J].