Seinat (Cucumis melo var. tibish) seeds were analyzed for their physiochemical properties and chemical composition of the oil in addition to antioxidant activity. The crude oil content was 31.1%, while the moisture, fiber, protein, ash and total sugar contents were 4.2%, 24.7%, 28.5%, 4.3%, and 6.9%, respectively. The main fatty acids were linoleic, oleic, palmitic, and stearic acids (61.10%, 18.75%, 10.37% and 9.18%, respectively). The total phenolic content was 28.17 mgg-' oil. Seinat seed oil also contained a good level of tocopherols; of which 8-tocopherol (63.43%) showed the highest content. P-sitosterol was found at 289 mg-100g-' oil of total sterols (302 mg100g-') in the oil. The crude oil showed a good antioxidant activity in four assays including reducing power, 3-carotene bleaching inhibition activity, ABTS and DPPH radical scavenging activities.