Implications for SARS-CoV-2 Vaccine Design: Fusion of Spike Glycoprotein Transmembrane Domain to Receptor-Binding Domain Induces Trimerization

被引：18
作者：
Azad, Taha [1 ,2 ]
Singaravelu, Ragunath [1 ,2 ]
Crupi, Mathieu J. F. [1 ,2 ]
Jamieson, Taylor [1 ,2 ]
Dave, Jaahnavi [1 ,2 ]
Brown, Emily E. F. [1 ,2 ]
Rezaei, Reza [1 ,2 ]
Taha, Zaid [1 ,2 ]
Boulton, Stephen [1 ,2 ]
Martin, Nikolas T. [1 ,2 ]
Surendran, Abera [1 ,2 ]
Poutou, Joanna [1 ,2 ]
Ghahremani, Mina [3 ]
Nouri, Kazem [4 ]
Whelan, Jack T. [1 ,2 ]
Duong, Jessie [1 ,2 ]
Tucker, Sarah [1 ,2 ]
Diallo, Jean-Simon [1 ,2 ]
Bell, John C. [1 ,2 ]
Ilkow, Carolina S. [1 ,2 ]
机构：
[1] Ottawa Hosp Res Inst, Ottawa, ON K1H 8L6, Canada
[2] Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada
[3] Univ Ottawa, Dept Biol, Ottawa, ON K1N 6N5, Canada
[4] Princess Margaret Canc Ctr, Univ Hlth Network, Toronto, ON M5G 2C1, Canada
来源：
MEMBRANES | 2020年 / 10卷 / 09期
基金：
加拿大健康研究院;
关键词：
trimerization; spike protein; receptor-binding domain; transmembrane domain; SARS-CoV-2; vaccine development; SEARCH;
D O I：
10.3390/membranes10090215
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic presents an urgent need for an effective vaccine. Molecular characterization of SARS-CoV-2 is critical to the development of effective vaccine and therapeutic strategies. In the present study, we show that the fusion of the SARS-CoV-2 spike protein receptor-binding domain to its transmembrane domain is sufficient to mediate trimerization. Our findings may have implications for vaccine development and therapeutic drug design strategies targeting spike trimerization. As global efforts for developing SARS-CoV-2 vaccines are rapidly underway, we believe this observation is an important consideration for identifying crucial epitopes of SARS-CoV-2.
引用
收藏
页码：1 / 8
页数：8
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