Quantification and Localization of Fucoidan in Laminaria japonica Using a Novel Antibody

被引：25

作者：

Mizuno, Masashi ^{[1
]}

Nishitani, Yosuke ^{[2
]}

Tanoue, Takeshi ^{[1
]}

Matoba, Yoshie ^{[1
]}

Ojima, Taka ^{[3
]}

Hashimoto, Takashi ^{[1
]}

Kanazawa, Kazuki ^{[1
]}

机构：

[1] Kobe Univ, Grad Sch Agr Sci, Kobe, Hyogo 6578501, Japan

[2] Kobe Univ, Org Adv Sci & Technol, Kobe, Hyogo 6578501, Japan

[3] Hokkaido Univ, Grad Sch Fisheries Sci, Hakodate, Hokkaido 0418611, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2009年 / 73卷 / 02期

关键词：

fucoidan; Laminaria japonica Areschoug; antibody; enzymed-linked immunosorbent assay (ELISA); localization; FUCUS-EVANESCENS; BROWN; CELLS;

D O I：

10.1271/bbb.80542

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The establishment of a simple technique to determine the concentration of fucoidan wag developed by using a monoclonal antibody against fucoidan. This antibody reacted with fucoidans purified from Laminaria japonica Areschoug (Makombu in Japanese) and Kjellmaniella gyrate Miyabe (Gagome), but not with polysaccharides from Undaria pinnatifida Suringar (Wakame). Neither laminarin nor algenic acid, which are constituents in Laminaria japonica, were recognized by the prepared antibody. Application of the enzymed-linked immunosorbent assay (ELISA) inhibition assay increased the specificity of fucoidan in measuring the fucoidan contents. On the basis of these results, it was ascertained that the ELISA inhibition assay of using the anti-fucoidan monoclonal antibody was rapid, accurate, and sensitive in measuring the content of fucoidan. In addition, the localization of fucoidan in Laminaria japonica was investigated. This is the first report of fucoidan being restricted to the outer cortical layer.

引用

页码：335 / 338

页数：4

共 18 条

[1] Structure of a fucoidan from the brown seaweed Fucus evanescens C.Ag. [J].