Green synthesis of silver nanoparticles by Phoma glomerata

被引:100
作者
Gade, Aniket [1 ]
Gaikwad, Swapnil [1 ]
Duran, Nelson [2 ,3 ]
Rai, Mahendra [1 ]
机构
[1] SGB Amravati Univ, Dept Biotechnol, Amravati 444602, Maharashtra, India
[2] Univ Fed ABC, Ctr Nat & Human Sci, Santo Andre, SP, Brazil
[3] Univ Estadual Campinas, Inst Chem, Biol Chem Lab, Campinas, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
Mycofabrication; Silver nanoparticles; Phoma glomerata; FTIR; TEM; BIOSYNTHESIS; MYCOSYNTHESIS;
D O I
10.1016/j.micron.2013.12.005
中图分类号
TH742 [显微镜];
学科分类号
摘要
We report an extracellular synthesis of silver nanoparticles (SNPs) by Phoma glomerate (MTCC-2210). The fungal filtrate showed rapid synthesis in bright sunlight. The Fourier transform infrared spectroscopy (FIR) revealed the presence of a protein cap on the silver nanoparticle, which leads to increase stability of SNP in the silver colloid. X-ray diffraction (XRD) analysis showed the number of Bragg's reflection, which are due to the face centered cubic structure of the crystalline SNPs. Transmission electron microscopy (TEM), scanning electron microscopy (SEM), nanoparticle tracking and analysis (NTA) demonstrated the synthesis of polydispersive and spherical SNPs. Energy dispersive X-ray spectroscopy (EDX) was used to confirm the elemental composition of the sample and Zeta potential measurement was carried out to determine the stability of mycofabricated SNPs. The alkaline pH, room temperature, sunlight demonstrated optimum synthesis. Apart from the physical conditions, concentration of silver nitrate and amount of fungal filtrate affects the mycofabrication process. The study of cultural and physical parameters during the mycofabrication of SNPs by P. glomerate will be helpful in order to increase the yield of mycofabricated SNPs of desired shape and size. The process of mycofabrication of SNPs by P. glomerata was found to be eco-friendly, safe and cost-effective nature. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:52 / 59
页数:8
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