The application of frequency-domain Fluorescence Lifetime Imaging Microscopy as a quantitative analytical tool for microfluidic devices

被引：35
作者：
Elder, A. D.
Matthews, S. M.
Swartling, J.
Yunus, K.
Frank, J. H.
Brennan, C. M.
Fisher, A. C.
Kaminski, C. F.
机构：
[1] Univ Cambridge, Dept Chem Engn, Cambridge CB2 3RA, England
[2] Sandia Natl Labs, Livermore, CA 94551 USA
[3] Syngenta Ltd, Huddersfield Mfg Ctr, Huddersfield HD2 1FF, W Yorkshire, England
来源：
OPTICS EXPRESS | 2006年 / 14卷 / 12期
关键词：
D O I：
10.1364/OE.14.005456
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We describe the application of wide-field frequency domain Fluorescence Lifetime Imaging Microscopy (FLIM) to imaging in microfluidic devices. FLIM is performed using low cost, intensity modulated Light Emitting Diodes (LEDs) for illumination. The use of lifetime imaging for quantitative analysis within such devices is demonstrated by mapping the molecular diffusion of iodide ions across a microchannel. (c) 2006 Optical Society of America.
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页码：5456 / 5467
页数：12
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