Co-production of α-amylase and β-galactosidase by Bacillus subtilis in complex organic substrates

被引：72

作者：

Konsoula, Zoe ^{[1
]}

Liakopoulou-Kyriakides, Maria ^{[1
]}

机构：

[1] Aristotle Univ Thessaloniki, Fac Chem Engn, Dept Chem, Thessaloniki 54124, Greece

来源：

BIORESOURCE TECHNOLOGY | 2007年 / 98卷 / 01期

关键词：

Bacillus subtilis; alpha-amylase; beta-galactosidase; thermostable; co-production; organic substrates;

D O I：

10.1016/j.biortech.2005.11.001

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Various nutrients belonging to three categories, carbon, organic nitrogen and complex organic sources, were investigated for the first time in terms of their effect on the co-production of extracellular thermostable alpha-amylase and beta-galactosidase by Bacillus subtilis, a bacterium isolated from fresh sheep's milk. Among the organic nitrogen sources tested, tryptone and corn steep liquor favored their production. Substitution of soluble starch by various starchy substrates, such as corn flour, had a positive effect on both enzyme yields. Furthermore, a two-fold higher production of both enzymes was achieved when corn steep liquor or tryptone was used in combination with the different flours. Among the divalent cations examined, calcium ions appeared to be vital for alpha-amylase production. The crude alpha-amylase and beta-galactosidase produced by this B. subtilis strain exhibited maximal activities at 135 degrees C and 65 degrees C, respectively, and were also found to be significantly stable at elevated temperatures. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：150 / 157

页数：8

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