Over-expression of rice R1-type MYB transcription factor confers different abiotic stress tolerance in transgenic Arabidopsis

Among various abiotic stresses, water deficit hit the first in the list followed by heavy metal stresses as a serious environmental growth-limiting factor that restricts the global crop yield. Molecular approaches will help us to trace key regulators which are involved in stress-related phenomenon to enhance crop productivity. The present study functionally characterized one of the key regulators, OsMYB-R1 in Arabidopsis. Phylogenetic analyses indicated that OsMYB-R1 had a close relationship with Sorghum bicolour and Zea mays. Ectopic expression of OsMYB-R1 in Arabidopsis resulted in improved tolerance to PEG/drought and chromium stress in addition to conferring no tolerance to salinity stress. Further RNA seq. data revealed that OsMYB-R1 regulates the expression of key genes that improve the root architecture and maintain the cellular homeostasis of transgenic lines through an efficient anti-oxidant system. It also reveals the differential gene expression of stress-responsive and hormone-responsive genes, which indicate the intricate network of defense regulatory machinery activated in transgenic lines. Additionally, salicylic acid (SA) plays a significant role in promoting the growth of the OsMYB-R1 over-expressing plants and increased GUS intensity in SA treated OsMYB-R1 promoter plants demonstrate the explicit role of SA signaling in overcoming stress tolerance. Whereas no significant change was observed in OsMYB-R1 over-expressing plants after ABA and MeJA treatment. Overall, OsMYB-R1 is a promising gene resource for improving abiotic stress tolerance in other crops, especially in dicotyledon plants.