Systems approach identifies TGA1 and TGA4 transcription factors as important regulatory components of the nitrate response of Arabidopsis thaliana roots

被引:270
作者
Alvarez, Jose M. [1 ,2 ]
Riveras, Eleodoro [1 ,2 ]
Vidal, Elena A. [1 ,2 ]
Gras, Diana E. [1 ,2 ]
Contreras-Lopez, Orlando [1 ,2 ]
Tamayo, Karem P. [1 ,2 ]
Aceituno, Felipe [1 ,2 ]
Gomez, Isabel [2 ]
Ruffel, Sandrine [3 ]
Lejay, Laurence [3 ]
Jordana, Xavier [2 ]
Gutierrez, Rodrigo A. [1 ,2 ]
机构
[1] Pontificia Univ Catolica Chile, Fac Ciencias Biol, FONDAP Ctr Genome Regulat, Dept Mol Genet & Microbiol, Santiago 8331150, Chile
[2] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Millennium Nucleus Ctr Plant Funct Gen, Dept Mol Genet & Microbiol, Santiago 8331150, Chile
[3] INRA, CNRS, SupAgro UM2,AgroM, Inst Biol Integrat Plantes,Unite Mixte Rech 5004, F-34060 Montpellier 2, France
关键词
Arabidopsis; TGA1 and TGA4; nitrate signal; lateral root growth; nitrate sensing; TRANSPORTER GENE; SIGNALING PATHWAYS; NITROGEN RESPONSES; GENOMIC ANALYSIS; EXPRESSION; NETWORK; METABOLISM; BIOLOGY; NRT2.1; ORGANIZATION;
D O I
10.1111/tpj.12618
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Nitrate acts as a potent signal to control global gene expression in Arabidopsis. Using an integrative bioinformatics approach we identified TGA1 and TGA4 as putative regulatory factors that mediate nitrate responses in Arabidopsis roots. We showed that both TGA1 and TGA4 mRNAs accumulate strongly after nitrate treatments in roots. Global gene expression analysis revealed 97% of the genes with altered expression in tga1tga4 double mutant plants respond to nitrate treatments, indicating that these transcription factors have a specific role in nitrate responses in Arabidopsis root organs. We found TGA1 and TGA4 regulate the expression of nitrate transporter genes NRT2.1 and NRT2.2. Specific binding of TGA1 to its cognate DNA sequence on NRT2.1 and NRT2.2 promoters was confirmed by chromatin immunoprecipitation assays. The tga1tga4 double mutant plants exhibit nitrate-dependent lateral and primary root phenotypes. Lateral root initiation is affected in both tga1tga4 and nrt1.2nrt2.2 double mutants, suggesting TGA1 and TGA4 regulate lateral root development at least partly via NRT2.1 and NRT2.2. Additional root phenotypes of tga1tga4 double mutants indicate that these transcription factors play an important role in root developmental responses to nitrate. These results identify TGA1 and TGA4 as important regulatory factors of the nitrate response in Arabidopsis roots.
引用
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页码:1 / 13
页数:13
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