Cell Patterning by Laser-Assisted Bioprinting

被引：58

作者：

Devillard, Raphael ^{[1
,2
]}

Pages, Emeline ^{[1
,2
]}

Correa, Manuela Medina ^{[1
,2
]}

Keriquel, Virginie ^{[1
,2
]}

Remy, Murielle ^{[1
,2
]}

Kalisky, Jerome ^{[1
,2
]}

Ali, Muhammad ^{[1
,2
]}

Guillotin, Bertrand ^{[1
,2
]}

Guillemot, Fabien ^{[1
,2
]}

机构：

[1] INSERM, U1026, Bordeaux, France

[2] Univ Bordeaux Segalen, Bordeaux, France

来源：

MICROPATTERNING IN CELL BIOLOGY, PT A | 2014年 / 119卷

关键词：

TISSUE; MICROARRAYS; ELASTICITY; PARAMETERS; MATRICES; GROWTH; SHAPE;

D O I：

10.1016/B978-0-12-416742-1.00009-3

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The aim of tissue engineering is to produce functional three-dimensional (3D) tissue substitutes. Regarding native organ and tissue complexity, cell density and cell spatial 3D organization, which influence cell behavior and fate, are key parameters in tissue engineering. Laser-Assisted Bioprinting (LAB) allows one to print cells and liquid materials with a cell- or picoliter-level resolution. Thus, LAB seems to be an emerging and promising technology to fabricate tissue-like structures that have the physiological functionality of their native counterparts. This technology has additional advantages such as automation, reproducibility, and high throughput. It makes LAB compatible with the (industrial) fabrication of 3D constructs of physiologically relevant sizes. Here we present exhaustively the numerous steps that allow printing of viable cells with a well-preserved micrometer pattern. To facilitate the understanding of the whole cell patterning experiment using LAB, it is discussed in two parts: (1) preprocessing: laser set-up, bio-ink cartridge and bio-paper preparation, and pattern design; and (2) processing: bio-ink printing on the bio-paper.

引用

页码：159 / 174

页数：16

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