Biodegradable electrospun nanofibers coated with platelet-rich plasma for cell adhesion and proliferation

被引：81

作者：

Diaz-Gomez, Luis ^{[1
,2
]}

Alvarez-Lorenzo, Carmen ^{[1
]}

Concheiro, Angel ^{[1
]}

Silva, Maite ^{[2
]}

Dominguez, Fernando ^{[3
]}

Sheikh, Faheem A. ^{[4
]}

Cantu, Travis ^{[4
]}

Desai, Raj ^{[4
]}

Garcia, Vanessa L. ^{[4
]}

Macossay, Javier ^{[4
]}

机构：

[1] Univ Santiago de Compostela, Fac Farm, Dept Farm & Tecnol Farmaceut, Santiago De Compostela 15872, Spain

[2] Univ Santiago de Compostela, Inst Ortopedia & Banco Tejidos Musculoesquelet, Santiago De Compostela 15872, Spain

[3] Fdn Publ Galega Med Xenom, Santiago De Compostela, Spain

[4] Univ Texas Pan Amer, Dept Chem, Edinburg, TX 78541 USA

来源：

MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2014年 / 40卷

关键词：

Electrospinning; PCL scaffold; Platelet-rich plasma; Cell proliferation; Angiogenesis; Growth factor sustained release; GROWTH-FACTORS; IN-VITRO; CHORIOALLANTOIC MEMBRANE; CONTROLLED-RELEASE; SCAFFOLDS; DELIVERY; DEGRADATION; BIOACTIVITY; HEPARIN;

D O I：

10.1016/j.msec.2014.03.065

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Biodegradable electrospun poly(e-caprolactone) (PCL) scaffolds were coated with platelet-rich plasma (PRP) to improve cell adhesion and proliferation. PRP was obtained from human buffy coat, and tested on human adipose-derived mesenchymal stem cells (MSCs) to confirm cell proliferation and cytocompatibility. Then, PRP was adsorbed on the PCL scaffolds via lyophilization, which resulted in a uniform sponge-like coating of 2.85 (S.D. 0.14) mg/mg. The scaffolds were evaluated regarding mechanical properties (Young's modulus, tensile stress and tensile strain), sustained release of total protein and growth factors (PDGF-BB, TGF-beta 1 and VEGF), and hemocompatibility. MSC seeded on the PRP-PCL nanofibers showed an increased adhesion and proliferation compared to pristine PCL fibers. Moreover, the adsorbed PRP enabled angiogenesis features observed as neovascularization in a chicken chorioallantoic membrane (CAM) model. Overall, these results suggest that PRP-PCL scaffolds hold promise for tissue regeneration applications. (c) 2014 Elsevier B.V. All rights reserved.

引用

页码：180 / 188

页数：9

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