Human micro-vascular endothelial cell seeding on Cr-DLC thin films for mechanical heart valve applications

被引：54
作者：
Ali, N. [1 ]
Kousar, Y.
Okpalugo, T. I.
Singh, V.
Pease, M.
Ogwu, A. A.
Gracio, J.
Titus, E.
Meletis, E. I.
Jackson, M. J.
机构：
[1] Univ Aveiro, Ctr Mech Technol & Automat, P-3800 Aveiro, Portugal
[2] Univ Ulster, NIBEC, Coleraine BT52 1SA, Londonderry, North Ireland
[3] Louisiana State Univ, Ctr Adv Microstruct & Devices, Baton Rouge, LA 70806 USA
[4] Univ Paisley, Thin Film Ctr, Paisley PA1 2BE, Renfrew, Scotland
[5] Univ Texas, Arlington, TX 76019 USA
[6] Purdue Univ, Dept Mech Engn, W Lafayette, IN 47907 USA
来源：
THIN SOLID FILMS | 2006年 / 515卷 / 01期
关键词：
endothelial cells; metal-DLC; haemocompatibility; heart valves;
D O I：
10.1016/j.tsf.2005.12.023
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
In this investigation, chromium modified diamond-like-carbon (Cr-DLC) films were studied for potential applications in mechanical heart valves. Three Cr-DLC samples were prepared using a magnetron sputtering technique employing an intensified plasma assisted processing (IPAP) system. The three samples consisted of the following Cr content: 1 at.%, 5 at.% and 10 at.%. The biological response of human micro-vascular endothelial cells (HMV-EC) seeded on Cr-DLC films was evaluated in terms of initial cell attachment and growth. The Cr-DLC films were characterized using Raman spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), secondary ion mass spectroscopy (SIMS) and by the contact angle technique. Endothelial cell adhesion and growth was found to be affected by changing the Cr content of Cr-DLC films. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：59 / 65
页数：7
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