Stimulation of Innate Immune Cells by Light-Activated TLR7/8 Agonists

被引:48
|
作者
Ryu, Keun Ah [1 ]
Stutts, Lalisa [1 ]
Tom, Janine K. [1 ]
Mancini, Rock J. [1 ]
Esser-Kahn, Aaron P. [1 ]
机构
[1] Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USA
关键词
TOLL-LIKE RECEPTORS; PATTERN-RECOGNITION; IMIDAZOQUINOLINES; R-848;
D O I
10.1021/ja412314j
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The innate immune response is controlled, in part, by the synergistic interaction of multiple Toll-like receptors (TLRs). This multi-receptor cooperation is responsible for the potent activity of many vaccines, but few tools have been developed to understand the spatio-temporal elements of TLR synergies. In this Communication, we present photo-controlled agonists of TLR7/8. By strategically protecting the active agonist moiety based on an agonist-bound crystal structure, TLR activity is suppressed and then regained upon exposure to light. We confirmed NF-kappa B production upon light exposure in a model macrophage cell line. Primary cell activity was confirmed by examining cytokine and cell surface marker production in bone-marrow-derived dendritic cells. Finally, we used light to activate dendritic cell sub-populations within a larger population.
引用
收藏
页码:10823 / 10825
页数:3
相关论文
共 50 条
  • [1] TLR7/8 agonists activate a mild immune response in rabbits through TLR8 but not TLR7
    Lai, Chao-Yang
    Liu, Yi-Ling
    Yu, Guann-Yi
    Maa, Ming-Chei
    Leu, Tzeng-Horng
    Xu, Congfeng
    Luo, Yunping
    Xian, Rong
    Chuang, Tsung-Hsien
    VACCINE, 2014, 32 (43) : 5593 - 5599
  • [2] Analysis of the neuroinflammatory response to TLR7 stimulation in the brain: Comparison of multiple TLR7 and/or TLR8 agonists
    Butchi, Niranjan B.
    Pourciau, Susan
    Du, Min
    Morgan, Tim W.
    Peterson, Karin E.
    JOURNAL OF IMMUNOLOGY, 2008, 180 (11): : 7604 - 7612
  • [3] Dissecting in vivo innate immune responses to TLR7/9 agonists in primates
    Puig, Montserrat
    Grajkowska, Lucja T.
    Mattapallil, Joseph J.
    Verthelyi, Daniela
    CYTOKINE, 2009, 48 (1-2) : 100 - 100
  • [4] Co-stimulation with TLR7/8 and TLR9 agonists induce down-regulation of innate immune responses in sheep blood mononuclear and B cells
    Booth, Jayaum S.
    Buza, Joram J.
    Potter, Andrew
    Babiuk, Lorne A.
    Mutwiri, George K.
    DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2010, 34 (05): : 572 - 578
  • [5] Porcine TLR8 and TLR7 are both activated by a selective TLR7 ligand, imiquimod
    Zhu, Jianzhong
    Lai, Ken
    Brownile, Robert
    Babiuk, Lorne A.
    Mutwiri, George K.
    MOLECULAR IMMUNOLOGY, 2008, 45 (11) : 3238 - 3243
  • [6] Interactions Between TLR7 and TLR9 Agonists and Receptors Regulate Innate Immune Responses by Astrocytes and Microglia
    NIRANJAN B. BUTCHI
    MIN DU
    KARIN E. PETERSON
    神经损伤与功能重建, 2010, (02) : 122 - 134
  • [7] Interactions Between TLR7 and TLR9 Agonists and Receptors Regulate Innate Immune Responses by Astrocytes and Microglia
    Butchi, Niranjan B.
    Du, Min
    Peterson, Karin E.
    GLIA, 2010, 58 (06) : 650 - 664
  • [8] TLR7 Agonists Display Potent Antiviral Effects against Norovirus Infection via Innate Stimulation
    Tuipulotu, Daniel Enosi
    Netzler, Natalie E.
    Lun, Jennifer H.
    Mackenzie, Jason M.
    White, Peter A.
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2018, 62 (05)
  • [9] Different innate immune profiles by astrocytes and microglia following TLR7 and/or TLR9 agonist stimulation
    Butchi, Niranjan B.
    Du, Min
    Karin, Peterson
    JOURNAL OF IMMUNOLOGY, 2009, 182
  • [10] STING and TLR7/8 agonists-based nanovaccines for synergistic antitumor immune activation
    Zhang, Bo-Dou
    Wu, Jun-Jun
    Li, Wen-Hao
    Hu, Hong-Guo
    Zhao, Lang
    He, Pei-Yang
    Zhao, Yu-Fen
    Li, Yan-Mei
    NANO RESEARCH, 2022, 15 (07) : 6328 - 6339