Ulvibacterium marinum gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae, isolated from a culture of the green alga Ulva prolifera

A Gram-stain negative, aerobic, rod-shaped, and non-motile bacterium, designated strain CCMM003(T), was isolated from a culture of the green alga Ulva prolifera. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CCMM003(T) belongs to the family Flavobacteriaceae and exhibits a close relationship to Pseudozobellia thermophila DSM 19858(T) (92.5%). Optimal growth occurred in the presence of 4% (w/v) NaCl, at pH 7.0 and 30 degrees C. The polar lipids of strain CCMM003(T) consisted of phosphatidylethanolamine and six unidentified lipids. The predominant isoprenoid quinone was MK-6. The major fatty acids were iso-C-15:0, iso-C-15:1 G, iso-C-17:0 3-OH and summed feature 3 (C(16:1)7c and/or iso-C-15:0 2-OH). The DNA G+C content of strain CCMM003(T) calculated on the basis of the genome sequence was 41.2mol% and the genome size was 5.9 Mbp. On the basis of data from this polyphasic study, strain CCMM003(T) is considered to represent a novel genus and species of the family Flavobacteriaceae, for which the name Ulvibacterium marinum gen. nov., sp. nov. is proposed. The type strain is CCMM003(T) (=MCCC 1K03244(T)=KCTC52639(T)).