Inhibition of Helicobacter pylori growth and its colonization factors by Parthenium hysterophorus extracts

被引:12
作者
Espinosa-Rivero, Jazmin [1 ]
Rendon-Huerta, Erika [2 ]
Romero, Irma [1 ]
机构
[1] Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Mexico City 04510, DF, Mexico
[2] Univ Nacl Autonoma Mexico, Fac Med, Dept Biol Celular & Tisular, Lab Inmunobiol, Mexico City 04510, DF, Mexico
关键词
Helicobacter pylori; Parthenium hysterophorus; Medicinal plant; Adherence; Urease; Motility; MEDICINAL-PLANTS; IN-VITRO; L; ADHESION; POLYSACCHARIDES; MOTILITY; CELLS;
D O I
10.1016/j.jep.2015.08.021
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Ethnopharmacological relevance: Parthenium hysterophorus (Asteraceae) is a traditional medicinal plant used to treat gastrointestinal disorders, such as gastritis. Helicobacter pylori have been described as the etiological agent of gastritis, peptic ulcer, as well as gastric adenocarcinoma. 50% of the world's population is infected with this bacterium and the current therapy fails due to the increment in antibiotic resistance; therefore, it is necessary to find new approaches to control H. pylori infection, either by its eradication or by preventing the bacterial colonization. Aim of the study: To investigate the effect of P. hysterophorus extracts on H. pylori growth and upon its colonization-related factors. Materials and methods: Five different polarity extracts from roots and aerial parts of P. hysterophorus were evaluated in vitro against H. pylori growth by the broth dilution method. Anti-colonization activities were determined as follows: motility in soft agar plates, urease activity by ammonia colorimetrical quantification, and adherence of FITC labeled H. pylori to AGS cells by fluorometrical measurement. Results: Organic extracts inhibited H. pylori growth. Particularly, the dichloromethane extract from roots showed a MIC of 15.6 mu g/ml while the aqueous extracts showed low or null activity. There is a direct correlation between antibacterial activity and inhibition of motility. Unease activity was partially inhibited by organic extracts, at best 46%, except for the roots dichloromethane extract which reached 74% of inhibition with 500 mu g/ml (IC50= 136.4 mu g/ml). Plant extracts inhibited adherence in different ranges but the dichloromethane-methanol ones possessed the highest effect, with a 70% maximal inhibition at 1 mg/ml. Conclusion: The results indicate that some P. hysterophorus extracts have various biological activities that could act synergistically against H. pylori. This work contributes to the ethnomedical knowledge of this species and underlines the potential of some organic extracts as a good source for the isolation of bioactive compounds. (c) 2015 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:253 / 260
页数:8
相关论文
共 51 条
[1]  
[Anonymous], MED USES C WEED PART
[2]  
Argueta V A., 1994, Atlas de las plantas de la medicina tradicional Mexicana
[3]  
Beil W., 2007, PHYTOMEDICINE, V14, P5
[4]  
Bucciarelli A, 2007, ARS PHARM, V48, P361
[5]   Helicobacter pylori moves through mucus by reducing mucin viscoelasticity [J].
Celli, Jonathan P. ;
Turner, Bradley S. ;
Afdhal, Nezam H. ;
Keates, Sarah ;
Ghiran, Ionita ;
Kelly, Ciaran P. ;
Ewoldt, Randy H. ;
McKinley, Gareth H. ;
So, Peter ;
Erramilli, Shyamsunder ;
Bansil, Rama .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (34) :14321-14326
[6]  
Cordell Geoffrey A., 1993, P195
[7]   Adherens junctions as targets of microorganisms: A focus on Helicobacter pylori [J].
Costa, Angela Margarida ;
Leite, Marina ;
Seruca, Raquel ;
Figueiredo, Ceu .
FEBS LETTERS, 2013, 587 (03) :259-265
[8]  
CSIR, 2001, WEALTH IND S, V7
[9]  
Das B., 1999, Fitoterapia, V70, P101, DOI 10.1016/S0367-326X(98)00014-8
[10]   EFFECT OF INHIBITION OF HELICOBACTER-PYLORI UREASE ACTIVITY BY ACETOHYDROXAMIC ACID ON SERUM GASTRIN IN DUODENAL-ULCER SUBJECTS [J].
ELNUJUMI, AM ;
DORRIAN, CA ;
CHITTAJALLU, RS ;
NEITHERCUT, WD ;
MCCOLL, KEL .
GUT, 1991, 32 (08) :866-870