Specificity of the ribosomal A site for aminoacyl-tRNAs

被引：25

作者：

Dale, Taraka ^{[2
]}

Fahlman, Richard P. ^{[3
]}

Olejniczak, Mikoaj ^{[4
]}

Uhlenbeck, Olke C. ^{[1
]}

机构：

[1] Northwestern Univ, Dept Biochem Mol Biol & Cell Biol, Evanston, IL 60208 USA

[2] Los Alamos Natl Lab, Biosci Div, Los Alamos, NM 87545 USA

[3] Univ Alberta, Dept Biochem, Edmonton, AB, Canada

[4] Polish Acad Sci, Inst Bioorgan Chem, Poznan, Poland

来源：

NUCLEIC ACIDS RESEARCH | 2009年 / 37卷 / 04期

基金：

美国国家卫生研究院;

关键词：

PEPTIDE-BOND FORMATION; ELONGATION-FACTOR TU; PROTEIN-SYNTHESIS; A-SITE; CODON RECOGNITION; MESSENGER-RNA; INDUCED-FIT; P-SITES; BINDING; SELECTION;

D O I：

10.1093/nar/gkn1040

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Although some experiments suggest that the ribosome displays specificity for the identity of the esterified amino acid of its aminoacyl-tRNA substrate, a study measuring dissociation rates of several misacylated tRNAs containing the GAC anticodon from the A site showed little indication for such specificity. In this article, an expanded set of misacylated tRNAs and two 2-deoxynucleotide-substituted mRNAs are used to demonstrate the presence of a lower threshold in k(off) values for aa-tRNA binding to the A site. When a tRNA binds sufficiently well to reach this threshold, additional stabilizing effects due to the esterified amino acid or changes in tRNA sequence are not observed. However, specificity for different amino acid side chains and the tRNA body is observed when tRNA binding is sufficiently weaker than this threshold. We propose that uniform aa-tRNA binding to the A site may be a consequence of a conformational change in the ribosome, induced by the presence of the appropriate combination of contributions from the anticodon, amino acid and tRNA body.

引用

页码：1202 / 1210

页数：9

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