Trichloroacetic acid treatment as a tricky way for rapid purification of 1N/4R tau protein

被引：6

作者：

Asadollahi, Kazem ^{[1
]}

Rafiee, Saharnaz ^{[1
]}

Riazi, Gholam Hossein ^{[1
]}

Pooyan, Shahriar ^{[1
]}

Afrasiabi, Ali ^{[1
]}

机构：

[1] Univ Tehran, IBB, Tehran, Iran

来源：

PROTEIN EXPRESSION AND PURIFICATION | 2016年 / 118卷

关键词：

4R/1N tau; Protein purification; Trichloroacetic acid; Perchloric acid; Differential precipitation; Heat treatment; PAIRED HELICAL FILAMENTS; IN-VITRO; ALZHEIMERS-DISEASE; AGGREGATION; DNA; PHOSPHORYLATION; MICROTUBULES; POLYMERIZATION; STABILIZATION; GLYCOSYLATION;

D O I：

10.1016/j.pep.2015.10.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tau protein consists of six different isoforms and each one has particular physiological roles. In order to analyze the specific function of each single isoforms, large quantity of highly purified tau isoforms is essential. Many studies have been done to purify tau isoforms by heat treatment, followed by perchloric acid and glycerol precipitation. We found out that 1N/4R tau is soluble in glycerol, that is why mentioned methods do not work for purifying this isoform. In this study, large amounts of active and highly purified (97%) 1N/4R tau protein has been prepared by utilization of trichloroacetic acid as precipitating agent. (C) 2015 Elsevier Inc. All rights reserved.

引用

收藏

页码：98 / 104

页数：7

相关论文

共 61 条

[11] PROJECTION DOMAINS OF MAP2 AND TAU DETERMINE SPACINGS BETWEEN MICROTUBULES IN DENDRITES AND AXONS [J].

CHEN, J ;

KANAI, Y ;

COWAN, NJ ;

HIROKAWA, N .

NATURE, 1992, 360 (6405) :674-676

[12] Anionic micelles and vesicles induce tau fibrillization in vitro [J].

Chirita, CN ;

Necula, M ;

Kuret, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (28) :25644-25650

[13] The microtubule-associated tau protein has intrinsic acetyltransferase activity [J].

Cohen, Todd J. ;

Friedmann, Dave ;

Hwang, Andrew W. ;

Marmorstein, Ronen ;

Lee, Virginia M. Y. .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2013, 20 (06) :756-+

[14] Uncovering the unfoldome: Enriching cell extracts for unstructured proteins by acid treatment [J].

Cortese, MS ;

Baird, JP ;

Uversky, VN ;

Dunker, AK .

JOURNAL OF PROTEOME RESEARCH, 2005, 4 (05) :1610-1618

[15] Phosphorylation of tau regulates its axonal transport by controlling its binding to kinesin [J].

Cuchillo-Ibanez, Inmaculada ;

Seereeram, Anjan ;

Byers, Helen L. ;

Leung, Kit-Yi ;

Ward, Malcolm A. ;

Anderton, Brian H. ;

Hanger, Diane P. .

FASEB JOURNAL, 2008, 22 (09) :3186-3195

[16] Role of the Tau N-terminal region in microtubule stabilization revealed by new endogenous truncated forms [J].

Derisbourg, Maxime ;

Leghay, Coline ;

Chiappetta, Giovanni ;

Fernandez-Gomez, Francisco-Jose ;

Laurent, Cyril ;

Demeyer, Dominique ;

Carrier, Sebastien ;

Buee-Scherrer, Valerie ;

Blum, David ;

Vinh, Joelle ;

Sergeant, Nicolas ;

Verdier, Yann ;

Buee, Luc ;

Hamdane, Malika .

SCIENTIFIC REPORTS, 2015, 5

[17] Tau isoform expression and regulation in human cortical neurons [J].

Deshpande, Atul ;

Win, Khin May ;

Busciglio, Jorge .

FASEB JOURNAL, 2008, 22 (07) :2357-2367

[18] Histone deacetylase 6 interacts with the microtubule-associated protein tau [J].

Ding, Huiping ;

Dolan, Philip J. ;

Johnson, Gail V. W. .

JOURNAL OF NEUROCHEMISTRY, 2008, 106 (05) :2119-2130

[19] Tau polymerization: Role of the amino terminus [J].

Gamblin, TC ;

Berry, RW ;

Binder, LI .

BIOCHEMISTRY, 2003, 42 (07) :2252-2257

[20] TURBIDIMETRIC STUDIES OF INVITRO ASSEMBLY AND DISASSEMBLY OF PORCINE NEUROTUBULES [J].

GASKIN, F ;

CANTOR, CR ;

SHELANSKI, ML .

JOURNAL OF MOLECULAR BIOLOGY, 1974, 89 (04) :737-+

← 1 2 3 4 5 6 7 →