Biofilm formation by a Bacillus subtilis strain that produces γ-polyglutamate

被引:103
作者
Morikawa, Masaaki
Kagihiro, Shinji
Haruki, Mitsuru
Takano, Kazufumi
Branda, Steve
Kolter, Roberto
Kanaya, Shigenori
机构
[1] Osaka Univ, Dept Mat & Life Sci, Grad Sch Design, Suita, Osaka 5650871, Japan
[2] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02215 USA
来源
MICROBIOLOGY-SGM | 2006年 / 152卷
关键词
GAMMA-POLY(GLUTAMIC ACID) FORMATION; LICHENIFORMIS; REGULATOR; GENE; SPORULATION;
D O I
10.1099/mic.0.29060-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The extracellular matrix produced by Bacillus subtilis B-1, an environmental strain that forms robust floating biofilms, was purified, and determined to be composed predominantly of gamma-polyglutamate (gamma-PGA), with a molecular mass of over 1000 kDa. Both biofilm formation and gamma-PGA production by B. subtilis B-1 increased with increasing Mn2+ or glycerol concentration. gamma-PGA was produced in a growth-associated manner in standing culture, and floating biofilms were formed. However, gamma-PGA was produced in a non-growth-associated manner in shaking culture conditions. When B. subtilis B-1 was grown in a microaerated culture system, floating biofilm formation and gamma-PGA production were significantly retarded, suggesting that oxygen depletion is involved in the initial steps of floating biofilm formation in standing culture. Proteomic analysis of membrane proteins demonstrated that flagellin, oligopeptide permease and Vpr protease precursor were the major proteins produced by cells in a floating biofilm and a colony.
引用
收藏
页码:2801 / 2807
页数:7
相关论文
共 31 条
[1]  
BOYD A, 1995, J IND MICROBIOL, V15, P162, DOI 10.1007/BF01569821
[2]   Biofilms:: the matrix revisited [J].
Branda, SS ;
Vik, Å ;
Friedman, L ;
Kolter, R .
TRENDS IN MICROBIOLOGY, 2005, 13 (01) :20-26
[3]   Fruiting body formation by Bacillus subtilis [J].
Branda, SS ;
González-Pastor, JE ;
Ben-Yehuda, S ;
Losick, R ;
Kolter, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (20) :11621-11626
[4]   Analysis of the lateral flagellar gene system of Aeromonas hydrophila AH-3 [J].
Canals, R ;
Altarriba, M ;
Vilches, S ;
Horsburgh, G ;
Shaw, JG ;
Tomás, JM ;
Merino, S .
JOURNAL OF BACTERIOLOGY, 2006, 188 (03) :852-862
[5]   Biofilm-defective mutants of Bacillus subtilis [J].
Chagneau, C ;
Saier, MH .
JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY, 2004, 8 (03) :177-188
[6]   Conservation of genes and processes controlled by the quorum response in bacteria:: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis [J].
Comella, N ;
Grossman, AD .
MOLECULAR MICROBIOLOGY, 2005, 57 (04) :1159-1174
[7]  
Cromwick AM, 1996, BIOTECHNOL BIOENG, V50, P222, DOI 10.1002/(SICI)1097-0290(19960420)50:2<222::AID-BIT10>3.0.CO
[8]  
2-P
[9]   EFFECTS OF MANGANESE(II) ON BACILLUS-LICHENIFORMIS ATCC-9945A PHYSIOLOGY AND GAMMA-POLY(GLUTAMIC ACID) FORMATION [J].
CROMWICK, AM ;
GROSS, RA .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1995, 17 (05) :259-267
[10]   Exopolysaccharide production is required for development of Escherichia coli K-12 biofilm architecture [J].
Danese, PN ;
Pratt, LA ;
Kolter, R .
JOURNAL OF BACTERIOLOGY, 2000, 182 (12) :3593-3596