Melatonin improves developmental competence of oocyte-granulosa cell complexes from porcine preantral follicles

被引：16

作者：

Cao, Zubing ^{[1
]}

Gao, Di ^{[1
]}

Tong, Xu ^{[1
]}

Xu, Tengteng ^{[1
]}

Zhang, Dandan ^{[1
]}

Wang, Yiqing ^{[1
]}

Liu, Ya ^{[1
]}

Li, Yunsheng ^{[1
]}

Zhang, Yunhai ^{[1
]}

Pu, Yong ^{[1
]}

机构：

[1] Anhui Agr Univ, Coll Anim Sci & Technol, Anhui Prov Key Lab Local Livestock & Poultry, Genet Resource Conservat & Breeding, Hefei 230036, Anhui, Peoples R China

来源：

THERIOGENOLOGY | 2019年 / 133卷

关键词：

Melatonin; Pig; Oocyte-granulosa cell complexes; Preantral follicles; Developmental competence; IN-VITRO DEVELOPMENT; BIDIRECTIONAL COMMUNICATION; CUMULUS CELLS; METABOLISM; MATURATION; PROMOTES; CULTURE; SIZE; EXPRESSION; SURVIVAL;

D O I：

10.1016/j.theriogenology.2019.05.003

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Melatonin has been reported to improve the survival rate of mouse and goat preantral follicles cultured in vitro. However, the role of melatonin in the development of oocyte-granulosa cell complexes (OGCs) isolated from preantral follicles remains unclear. Cumulus-oocyte complexes were isolated from OGCs cultured in vitro for 18.5 days and were then maturated in vitro for 42 h. The matured oocytes were parthenogenetically activated and were further cultured up to the blastocyst stage. We found that the developmental capacity of oocytes from in vitro cultured OGCs was significantly inferior to that from in vivo grown counterparts. Additionally, a 10(-5) M dose of melatonin added to the medium during in vitro culture of OGCs did not improve oocyte meiotic maturation but enhanced blastocyst rate of partheno-genetically activated embryos. Besides, these beneficial effects could be reversed by luzindole treatment, a melatonin membrane receptor antagonist. mRNA sequencing analysis further revealed that melatonin caused differential expression of 76 genes of which 75 were upregulated and 1 was downregulated in OGCs. Twelve of the 76 genes were identified as potential regulators of metabolic pathways by functional analysis. Taken together, these results indicate that melatonin improves developmental competence of porcine oocyte-granulosa cell complexes. (C) 2019 Published by Elsevier Inc.

引用

页码：149 / 158

页数：10

共 48 条

[1] Melatonin has dose-dependent effects on folliculogenesis, oocyte maturation capacity and steroidogenesis [J].