Mass Spectrometric Proteomics Reveals that Nuclear Protein Positive Cofactor PC4 Selectively Binds to Cross-Linked DNA by a trans-Platinum Anticancer Complex

被引：33

作者：

Du, Zhifeng ^{[1
]}

Luo, Qun ^{[1
]}

Yang, Liping ^{[2
]}

Bing, Tao ^{[1
]}

Li, Xianchan ^{[1
]}

Guo, Wei ^{[1
]}

Wu, Kui ^{[1
]}

Zhao, Yao ^{[1
]}

Xiong, Shaoxiang ^{[1
]}

Shangguan, Dihua ^{[1
]}

Wang, Fuyi ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, CAS Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China

[2] Nantong Univ, Tumor Hosp, Canc Res Ctr, Nantong 226361, Jiangsu, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2014年 / 136卷 / 08期

关键词：

RECOGNITION; ANTITUMOR; LIGANDS; REPAIR;

D O I：

10.1021/ja410678y

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An MS-based proteomic strategy combined with chemically functionalized gold nanoparticles as affinity probes was developed and validated by successful identification and quantification of HMGB1, which is well characterized to interact selectively with 1,2-cross-linked DNA by cisplatin, from whole cell lysates. The subsequent application of this method to identify proteins responding to 1,3-cross-linked DNA by a trans-platinum anticancer complex, trans-PtTz (Tz = thiazole), revealed that the human nuclear protein positive cofactor PC4 selectively binds to the damaged DNA, implying that PC4 may play a role in cellular response to DNA damage by trans-PtTz.

引用

页码：2948 / 2951

页数：4

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