The scaffold protein MUPP1 regulates odorant-mediated signaling in olfactory sensory neurons

被引：14

作者：

Baumgart, Sabrina ^{[1
]}

Jansen, Fabian ^{[1
]}

Bintig, Willem ^{[2
]}

Kalbe, Benjamin ^{[1
]}

Herrmann, Christian ^{[3
]}

Klumpers, Fabian ^{[3
]}

Koester, S. David ^{[3
]}

Scholz, Paul ^{[1
]}

Rasche, Sebastian ^{[1
]}

Dooley, Ruth ^{[4
]}

Metzler-Nolte, Nils ^{[5
]}

Spehr, Marc ^{[6
]}

Hatt, Hanns ^{[1
]}

Neuhaus, Eva M. ^{[2
,7
]}

机构：

[1] Ruhr Univ Bochum, Fac Biol & Biotechnol, D-44780 Bochum, Germany

[2] Charite, Cluster Excellence NeuroCure, D-10117 Berlin, Germany

[3] Ruhr Univ Bochum, D-44780 Bochum, Germany

[4] Beaumont Hosp, Royal Coll Surg Ireland, Dept Mol Med, Dublin 9, Ireland

[5] Ruhr Univ Bochum, Chair Inorgan Chem Bioinorgan Chem 1, D-44780 Bochum, Germany

[6] Rhein Westfal TH Aachen, Inst Biol 2, Dept Chemosensat, D-52074 Aachen, Germany

[7] Univ Hosp Jena, D-07743 Jena, Germany

来源：

JOURNAL OF CELL SCIENCE | 2014年 / 127卷 / 11期

关键词：

MUPP1; PDZ; Olfactory neuron; Scaffolding protein; PDZ DOMAIN PROTEIN; RECEPTOR NEURONS; RESPONSE TERMINATION; CHANNEL; CILIA; AMPLIFICATION; TRANSDUCTION; COMPLEX; SENSITIVITY; ADAPTATION;

D O I：

10.1242/jcs.144220

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The olfactory signal transduction cascade transforms odor information into electrical signals by a cAMP-based amplification mechanism. The mechanisms underlying the very precise temporal and spatial organization of the relevant signaling components remains poorly understood. Here, we identify, using co-immunoprecipitation experiments, a macromolecular assembly of signal transduction components in mouse olfactory neurons, organized through MUPP1. Disruption of the PDZ signaling complex, through use of an inhibitory peptide, strongly impaired odor responses and changed the activation kinetics of olfactory sensory neurons. In addition, our experiments demonstrate that termination of the response is dependent on PDZ-based scaffolding. These findings provide new insights into the functional organization, and regulation, of olfactory signal transduction.

引用

页码：2518 / 2527

页数：10

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