The xanthophyll cycle pigments, violaxanthin and zeaxanthin, modulate molecular organization of the photosynthetic antenna complex LHCII

被引:11
|
作者
Janik, Ewa [1 ]
Bednarska, Joanna [1 ]
Zubik, Monika [1 ]
Sowinski, Karol [1 ,2 ]
Luchowski, Rafal [1 ]
Grudzinski, Wojciech [1 ]
Matosiuk, Dariusz [2 ]
Gruszecki, Wieslaw I. [1 ]
机构
[1] Marie Curie Sklodowska Univ, Inst Phys, Dept Biophys, PL-20031 Lublin, Poland
[2] Med Univ Lublin, Fac Pharm, Lublin, Poland
关键词
Photosynthesis; LHCII; Chlorophyll fluorescence; Xanthophyll cycle; Violaxanthin; Zeaxanthin; LIGHT-HARVESTING COMPLEX; ENERGY-DISSIPATION; PHOTOSYSTEM-II; CHLOROPHYLL FLUORESCENCE; INDUCED ISOMERIZATION; THYLAKOID MEMBRANES; LIPID-MEMBRANES; PLANTS; AGGREGATION; STATE;
D O I
10.1016/j.abb.2016.01.003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effect of violaxanthin and zeaxanthin, two main carotenoids of the xanthophyll cycle, on molecular organization of LHCII, the principal photosynthetic antenna complex of plants, was studied in a model system based on lipid-protein membranes, by means of analysis of 77 K chlorophyll a fluorescence and "native" electrophoresis. Violaxanthin was found to promote trimeric organization of LHCII, contrary to zeaxanthin which was found to destabilize trimeric structures. Moreover, violaxanthin was found to induce decomposition of oligomeric LHCII structures formed in the lipid phase and characterized by the fluorescence emission band at 715 nm. Both pigments promoted formation of two-component supra molecular structures of LHCII and xanthophylls. The violaxanthin-stabilized structures were composed mostly of LHCII trimers while, the zeaxanthin-stabilized supramolecular structures of LHCII showed more complex organization which depended periodically on the xanthophyll content. The effect of the xanthophyll cycle pigments on molecular organization of LHCII was analyzed based on the results of molecular modeling and discussed in terms of a physiological meaning of this mechanism. Supramolecular structures of LHCII stabilized by violaxanthin, prevent uncontrolled oligomerization of LHCII, potentially leading to excitation quenching, therefore can be considered as structures protecting the photosynthetic apparatus against energy loses at low light intensities. (C) 2016 Elsevier Inc. All rights reserved.
引用
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页码:1 / 9
页数:9
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