Identification of internal control genes for gene expression studies in olive mesocarp tissue during fruit ripening

被引:8
作者
Ben Ali, S. [1 ,2 ,3 ]
Guasmi, F. [2 ]
Mohamed, M. B. [1 ,2 ,3 ]
Benhaj, K. [4 ]
Boussora, F. [2 ]
Triki, T. [2 ]
Kammoun, N. G. [1 ]
机构
[1] Inst Olive, Unit Technol & Qual, Sfax, Tunisia
[2] Inst Arid Land, Lab Arid Land, Medenine, Tunisia
[3] Fac Sci, Bizerte, Tunisia
[4] High Inst Appl Biotechnol, Medenine, Tunisia
关键词
Fruit development; Gene expression; Olive; Reference gene; RT-qPCR; REAL-TIME PCR; MIQE GUIDELINES; RT-QPCR; NORMALIZATION; ARABIDOPSIS; SELECTION;
D O I
10.1016/j.sajb.2018.04.018
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Olive (Olea europaea L.) is one of the most important fruit trees in the Mediterranean zone. For olive gene expression studies, reverse transcriptase quantitative polymerase chain reaction (RT-ciPCR) is the most used and chosen technique in addition to the use of appropriate reference genes. So in this context, in our research, we analyzed six different genes of control in order to select the most stable one during olive fruit development. We used ten samples from five periods of fruit maturity of two different olive cultivars "Chemlali Sfax" and "Chetour during the year 2016 The profiles of expression of studied genes were assessed in three replicates. To analyze the results, we tried to use two Excel-based software: GeNorm and Bestkeeper. It results that software packages selected Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Elongation factor-2alpha (EF-2a) as stable gene in Chetoui and across cultivars. GAPDH and ACTT were found as the steadiest ones in Chemlali Sfax. Our study suggests the use of both GAPDH and EF-2a as two reference genes for the normalizing RT-qPCR data in future researches of olive fruit tissue development. (C) 2018 SAAB. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:11 / 16
页数:6
相关论文
共 50 条
[41]   Identification of reference genes for studies of quantitative gene expression in male and female quail tissues [J].
Macario, Maise dos Santos ;
Nascimento, C. S. ;
Sousa, F. C. B. ;
Oliveira, I. R. S. ;
Vesco, A. P. D. ;
Barbosa, L. T. ;
Sousa, K. R. S. .
ANIMAL BIOTECHNOLOGY, 2023, 34 (07) :2400-2413
[42]   Evaluation of RNA Extraction Methods and Identification of Putative Reference Genes for Real-Time Quantitative Polymerase Chain Reaction Expression Studies on Olive (Olea europaea L.) Fruits [J].
Nonis, Alberto ;
Vezzaro, Alice ;
Ruperti, Benedetto .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2012, 60 (27) :6855-6865
[43]   Identification of Internal Reference Genes for Gene Expression Normalization between the Two Sexes in Dioecious White Campion [J].
Zemp, Niklaus ;
Minder, Aria ;
Widmer, Alex .
PLOS ONE, 2014, 9 (03)
[44]   Validation of Endogenous Control Genes by Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction for Acute Leukemia Gene Expression Studies [J].
Pessoa, Flavia Melo Cunha de Pinho ;
Viana, Vitoria Beatriz de Jesus ;
de Oliveira, Marcelo Braga ;
Nogueira, Beatriz Maria Dias ;
Ribeiro, Rodrigo Monteiro ;
Oliveira, Deivide de Sousa ;
Lopes, Germison Silva ;
Vieira, Ricardo Parente Garcia ;
de Moraes Filho, Manoel Odorico ;
de Moraes, Maria Elisabete Amaral ;
Khayat, Andre Salim ;
Moreira, Fabiano Cordeiro ;
Moreira-Nunes, Caroline Aquino .
GENES, 2024, 15 (02)
[45]   Comprehensive analysis of plastid gene expression during fruit development and ripening of kiwifruit [J].
Chen, Qiqi ;
Shen, Pan ;
Bock, Ralph ;
Li, Shengchun ;
Zhang, Jiang .
PLANT CELL REPORTS, 2022, 41 (04) :1103-1114
[46]   Comprehensive analysis of plastid gene expression during fruit development and ripening of kiwifruit [J].
Qiqi Chen ;
Pan Shen ;
Ralph Bock ;
Shengchun Li ;
Jiang Zhang .
Plant Cell Reports, 2022, 41 :1103-1114
[47]   Banana sucrose-phosphate synthase gene expression during fruit ripening [J].
doNascimento, JRO ;
Cordenunsi, BR ;
Lajolo, FM ;
Alcocer, MJC .
PLANTA, 1997, 203 (03) :283-288
[48]   Characterization and expression of abscisic acid signal transduction genes during mulberry fruit ripening [J].
Zhu, Panpan ;
Cai, Yuxiang ;
Yu, Jian ;
Zhao, Aichun ;
Liang, Yanmei ;
Liu, Changying ;
Wang, Chuanhong ;
Yu, Maode .
ACTA PHYSIOLOGIAE PLANTARUM, 2017, 39 (07)
[49]   Ethylene and developmental signals regulate expression of lipoxygenase genes during tomato fruit ripening [J].
Griffiths, A ;
Barry, C ;
Alpuche-Solis, AG ;
Grierson, D .
JOURNAL OF EXPERIMENTAL BOTANY, 1999, 50 (335) :793-798
[50]   Changes in the expression of ADP-glucose pyrophosphorylase genes during fruit ripening in strawberry [J].
Park, Jeong-Il ;
Kim, In-Jung .
FOOD SCIENCE AND BIOTECHNOLOGY, 2007, 16 (03) :343-348