Localization of Crystalline Allomorphs in Cellulose Microfibril

被引:42
作者
Horikawa, Yoshiki [1 ]
Sugiyama, Junji [1 ]
机构
[1] Kyoto Univ, Res Inst Sustainable Humanosphere, Kyoto 6110011, Japan
关键词
HYDROGEN-BONDING SYSTEM; SYNCHROTRON X-RAY; I-ALPHA; NATIVE CELLULOSE; ELECTRON-DIFFRACTION; BETA; TRANSFORMATION; PHASES;
D O I
10.1021/bm900413k
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report an FTIR spectroscopic technique combined with intracrystalline deuteration and rehydrogenation of cellulose samples to investigate the localization of I-alpha. and I-beta domains within a cellulose microfibril obtained from I-alpha-rich algae. When Glaucocystis cellulose incompletely converted from I-alpha to I-beta was deuterated and rehydrogenated at elevated temperature, OD groups involved in hydrogen bonding in the I-beta domain first reverted to OH, followed by those in the I-alpha domain, Suggesting that the I-alpha core domain was surrounded by the I-beta domain in an artificially induced sample. We concluded that this I-alpha -> I-beta conversion proceeded from the Surface toward the core. Native celluloses from Valonia and Cladophora were first deuterated without changing the allomorphic composition, and rehydrogenation was studied from I-alpha- and I-beta-specific absorbances. Surprisingly, both absorbances changed synchronously, clearly indicating that the simple "skin-core" distribution model of I-alpha and I-beta domains is not realistic at least for these native celluloses.
引用
收藏
页码:2235 / 2239
页数:5
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