GD3 synthase overexpression enhances proliferation and migration of MDA-MB-231 breast cancer cells

被引:54
作者
Cazet, Aurelie [1 ]
Groux-Degroote, Sophie [1 ]
Teylaert, Beatrice [1 ]
Kwon, Kyung-Min [2 ]
Lehoux, Sylvain [1 ]
Slomianny, Christian [3 ]
Kim, Cheorl-Ho [2 ]
Le Bourhis, Xuefen [4 ]
Delannoy, Philippe [1 ]
机构
[1] Univ Sci & Technol Lille, CNRS, Struct & Funct Glycobiol Unit, UMR 8576, F-59655 Villeneuve Dascq, France
[2] Sungkyunkwan Univ, Dept Biol Sci, Mol & Cellular Glycobiol Unit, Suwon, Kyunggi Do, South Korea
[3] Univ Sci & Technol Lille, INSERM, IFR 147, U800, F-59655 Villeneuve Dascq, France
[4] Univ Sci & Technol Lille, INSERM, U908, F-59655 Villeneuve Dascq, France
关键词
breast cancer; cell proliferation; gangliosides; G(D3) synthase; MDA-MB-231; GANGLIOSIDE GD3; GENE-EXPRESSION; SIALYL-TN; SIALYLTRANSFERASE EXPRESSION; CARBOHYDRATE ANTIGENS; TUMOR MALIGNANCY; PROGNOSTIC VALUE; MESSENGER-RNA; GROWTH; ALPHA-2,8-SIALYLTRANSFERASE;
D O I
10.1515/BC.2009.054
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The disialoganglioside G(D3) is an oncofetal marker of a variety of human tumors including melanoma and neuroblastoma, playing a key role in tumor progression. G(D3) and 9-O-acetyl-G(D3) are overexpressed in approximately 50% of invasive ductal breast carcinoma, but no relationship has been established between disialoganglioside expression and breast cancer progression. In order to determine the effect of G(D3) expression on breast cancer development, we analyzed the biosynthesis of gangliosides in several breast epithelial cell lines including MDA-MB-231, MCF-7, BT-20, T47-D, and MCF10A, by immunocytochemistry, flow cytometry, and real-time PCR. Our results show that, in comparison to tumors, cultured breast cancer cells express a limited pattern of gangliosides. Disialogangliosides were not detected in any cell line and G(M3) was only observed at the cell surface of MDA-MB-231 cells. To evaluate the influence of G(D3) in breast cancer cell behavior, we established and characterized MDA-MB-231 cells overexpressing G(D3) synthase. We show that G(D3) synthase expressing cells accumulate G(D3), G(D2), and G(T3) at the cell surface. Moreover, G(D3) synthase overexpression bypasses the need of serum for cell growth and increases cell migration. This suggests that G(D3) synthase overexpression may contribute to increasing the malignant properties of breast cancer cells.
引用
收藏
页码:601 / 609
页数:9
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