Genetic diversity of Anaplasma marginale in calves with anaplasmosis on farms in Minas Gerais, Brazil

被引:5
作者
Bahia, Michele [1 ]
da Silva, Lucas Teixeira [2 ]
da Silva, Bruno Montijo [2 ]
Cordeiro, Matheus dias [1 ]
Guterres, Alexandro [3 ]
da Silva, Claudia Bezerra [1 ]
da Silva, Jenevaldo Barbosa [2 ]
Fonseca, Adivaldo Henrique [1 ]
机构
[1] Univ Fed Rural Rio de Janeiro, Vet Inst, Dept Epidemiol & Publ Hlth, Br 465,Km 7, BR-23897000 Seropedica, RJ, Brazil
[2] Fed Univ Jequetinhonha & Mucuri Valleys, Inst Agr Sci, Teofilo Otoni, Brazil
[3] Fiocruz MS, Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Hantaviroses & Rickettsioses Lab, Rio De Janeiro, Brazil
关键词
Anaplasmosis; msp1a; Strains; Rickettsia; Genotyping; RIO-DE-JANEIRO; MOLECULAR PHYLOGENY; BOVINE ANAPLASMOSIS; SEQUENCE ALIGNMENT; TRANSMISSION; STRAINS; CATTLE; STATE; RICKETTSIALES; MSP1-ALPHA;
D O I
10.1016/j.ttbdis.2020.101552
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The objective of this study was to identify Anaplasma marginale strains in dairy heifers from farms with a history of anaplasmosis in the northwest region of the State of Minas Gerais, Brazil. Among the examined animals of the four farms, the overall prevalence total of A. marginale was 55.7 % for gene msp5 and 36.7 % for blood smear. Thirty DNA samples (from 24 asymptomatic and six symptomatic animals) positive for A. marginale msp1 alpha were sequenced to study genotype and strain diversity. The majority (28/30) were the E genotype, followed by C (1/30) and G (1/30). Thirteen different strains were found: alpha-beta-F-F-F (nine animals), 13-27-27 (three animals), tau-27-18 (three animals), alpha-beta-beta- BRA1-31 (three animals), alpha-22-1318 (three animals), 80-F-F- F-F (three animals), and alpha -22-13-13, alpha-beta-beta-Gamma, M-phi-phi-phi-phi-F, 42-25- 25-31, Q-Q-Q-M, B-Q-B-Q-B-M, and 16-17-F-F (one animal each). A new structure repeated in tandem was described and named BRA 1 (TDSSSASGVLSQSGQASTSSQLG). The alpha-beta-F-F-F strain was present in all animals with acute anaplasmosis and in three animals asymptomatic. Thus, although 13 strains were observed in the animals evaluated, only the alpha-beta-F-F-F strain was identified during occurrence of acute disease and mortality, we suggest that this strain has important pathogenicity for calves in northeastern Minas Gerais.
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页数:6
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